Forskning ved Københavns Universitet - Københavns Universitet

Forside

Integrated mapping of pharmacokinetics and pharmacodynamics in a patient-derived xenograft model of glioblastoma

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningfagfællebedømt

  • Elizabeth C Randall
  • Janice K Laramy
  • Minjee Kim
  • Alison Roos
  • David Calligaris
  • Michael S Regan
  • Shiv K Gupta
  • Ann C Mladek
  • Brett L Carlson
  • Aaron J Johnson
  • Fa-Ke Lu
  • X Sunney Xie
  • Brian A Joughin
  • Raven J Reddy
  • Sen Peng
  • Walid M Abdelmoula
  • Pamela R Jackson
  • Aarti Kolluri
  • Katherine A Kellersberger
  • Jeffrey N Agar
  • Douglas A Lauffenburger
  • Kristin R Swanson
  • Nhan L Tran
  • William F Elmquist
  • Forest M White
  • Jann N Sarkaria
  • Nathalie Y R Agar

Therapeutic options for the treatment of glioblastoma remain inadequate despite concerted research efforts in drug development. Therapeutic failure can result from poor permeability of the blood-brain barrier, heterogeneous drug distribution, and development of resistance. Elucidation of relationships among such parameters could enable the development of predictive models of drug response in patients and inform drug development. Complementary analyses were applied to a glioblastoma patient-derived xenograft model in order to quantitatively map distribution and resulting cellular response to the EGFR inhibitor erlotinib. Mass spectrometry images of erlotinib were registered to histology and magnetic resonance images in order to correlate drug distribution with tumor characteristics. Phosphoproteomics and immunohistochemistry were used to assess protein signaling in response to drug, and integrated with transcriptional response using mRNA sequencing. This comprehensive dataset provides simultaneous insight into pharmacokinetics and pharmacodynamics and indicates that erlotinib delivery to intracranial tumors is insufficient to inhibit EGFR tyrosine kinase signaling.

OriginalsprogEngelsk
TidsskriftNature Communications
Vol/bind9
Udgave nummer1
Sider (fra-til)4904
ISSN2041-1723
DOI
StatusUdgivet - 21 nov. 2018
Eksternt udgivetJa

ID: 209323322