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Integrative promoter cloning plasmid vectors for Rhizobium meliloti

Publikation: Bidrag til tidsskriftTidsskriftartikel

  • P Elö
  • S Semsey
  • A Kereszt
  • T Nagy
  • P Papp
  • L Orosz

A set of integrative 'promoter probe' plasmids were constructed for both translational and transcriptional fusions. The vectors are based on the broad host range, low copy number plasmid pRK290 (IncPl) in which the attachment site of Rhizobium phage 16-3 and the lacZ gene of Escherichia coli were combined. The vectors integrate into the chromosome of Rhizobium meliloti, providing also the advantages of the single copy promoter probe cassettes. Thus they fulfil the prerequisite of the systems used for investigating gene regulation. The plasmids were applied for the study of the transcription regulation of the 16-3 phage. Their versatile use is also demonstrated.

OriginalsprogEngelsk
TidsskriftF E M S Microbiology Letters
Vol/bind159
Udgave nummer1
Sider (fra-til)7-13
Antal sider7
ISSN0378-1097
DOI
StatusUdgivet - 1 feb. 1998
Eksternt udgivetJa

ID: 163918158