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Interleukin 1-induced down-regulation of antibody binding to CD4 molecules on human lymphocytes

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningfagfællebedømt

Standard

Interleukin 1-induced down-regulation of antibody binding to CD4 molecules on human lymphocytes. / Tvede, N; Christensen, L D; Ødum, Niels; Wiik, A; Bendtzen, K.

I: Scandinavian Journal of Immunology, Bind 27, Nr. 6, 1988, s. 679-84.

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningfagfællebedømt

Harvard

Tvede, N, Christensen, LD, Ødum, N, Wiik, A & Bendtzen, K 1988, 'Interleukin 1-induced down-regulation of antibody binding to CD4 molecules on human lymphocytes', Scandinavian Journal of Immunology, bind 27, nr. 6, s. 679-84.

APA

Tvede, N., Christensen, L. D., Ødum, N., Wiik, A., & Bendtzen, K. (1988). Interleukin 1-induced down-regulation of antibody binding to CD4 molecules on human lymphocytes. Scandinavian Journal of Immunology, 27(6), 679-84.

Vancouver

Tvede N, Christensen LD, Ødum N, Wiik A, Bendtzen K. Interleukin 1-induced down-regulation of antibody binding to CD4 molecules on human lymphocytes. Scandinavian Journal of Immunology. 1988;27(6):679-84.

Author

Tvede, N ; Christensen, L D ; Ødum, Niels ; Wiik, A ; Bendtzen, K. / Interleukin 1-induced down-regulation of antibody binding to CD4 molecules on human lymphocytes. I: Scandinavian Journal of Immunology. 1988 ; Bind 27, Nr. 6. s. 679-84.

Bibtex

@article{f3d0c3d0fda111ddb219000ea68e967b,
title = "Interleukin 1-induced down-regulation of antibody binding to CD4 molecules on human lymphocytes",
abstract = "Interleukin 1 (IL-1) is involved in the early activation of T lymphocytes. The CD4 antigen, described as a phenotypic marker of helper T cells, is also important in early T-cell activation by its ability to bind to MHC class II molecules on antigen-presenting cells, and to transmit positive (and negative) signals to the cells. We observed that purified human monocyte IL-1 as well as recombinant IL-1 alpha and IL-1 beta selectively decreased the binding of monoclonal antibodies to CD4 on the surface of otherwise unstimulated blood T cells, in contrast to prestimulated and continuously grown CD4+ cells. Under optimal growth conditions, the initial reduction in antibody binding to CD4 was followed by an apparent re-expression of the CD4 antigen even in the presence of high concentrations of IL-1. This re-expression did not occur if the cells were cultured at 4 degrees C, or after treatment with actinomycin D or cytochalasin B, indicating that protein synthesis and intact microfilament function were essential for re-expression of CD4 binding. The mechanism by which CD4 molecules are physically and/or functionally modulated by IL-1 is unclear.",
author = "N Tvede and Christensen, {L D} and Niels {\O}dum and A Wiik and K Bendtzen",
note = "Keywords: Antibodies, Monoclonal; Antigen-Antibody Reactions; Antigens, Differentiation, T-Lymphocyte; Flow Cytometry; Humans; Interleukin-1; Isoantibodies; Lymphocyte Activation; T-Lymphocytes",
year = "1988",
language = "English",
volume = "27",
pages = "679--84",
journal = "Scandinavian Journal of Immunology",
issn = "0300-9475",
publisher = "Wiley-Blackwell",
number = "6",

}

RIS

TY - JOUR

T1 - Interleukin 1-induced down-regulation of antibody binding to CD4 molecules on human lymphocytes

AU - Tvede, N

AU - Christensen, L D

AU - Ødum, Niels

AU - Wiik, A

AU - Bendtzen, K

N1 - Keywords: Antibodies, Monoclonal; Antigen-Antibody Reactions; Antigens, Differentiation, T-Lymphocyte; Flow Cytometry; Humans; Interleukin-1; Isoantibodies; Lymphocyte Activation; T-Lymphocytes

PY - 1988

Y1 - 1988

N2 - Interleukin 1 (IL-1) is involved in the early activation of T lymphocytes. The CD4 antigen, described as a phenotypic marker of helper T cells, is also important in early T-cell activation by its ability to bind to MHC class II molecules on antigen-presenting cells, and to transmit positive (and negative) signals to the cells. We observed that purified human monocyte IL-1 as well as recombinant IL-1 alpha and IL-1 beta selectively decreased the binding of monoclonal antibodies to CD4 on the surface of otherwise unstimulated blood T cells, in contrast to prestimulated and continuously grown CD4+ cells. Under optimal growth conditions, the initial reduction in antibody binding to CD4 was followed by an apparent re-expression of the CD4 antigen even in the presence of high concentrations of IL-1. This re-expression did not occur if the cells were cultured at 4 degrees C, or after treatment with actinomycin D or cytochalasin B, indicating that protein synthesis and intact microfilament function were essential for re-expression of CD4 binding. The mechanism by which CD4 molecules are physically and/or functionally modulated by IL-1 is unclear.

AB - Interleukin 1 (IL-1) is involved in the early activation of T lymphocytes. The CD4 antigen, described as a phenotypic marker of helper T cells, is also important in early T-cell activation by its ability to bind to MHC class II molecules on antigen-presenting cells, and to transmit positive (and negative) signals to the cells. We observed that purified human monocyte IL-1 as well as recombinant IL-1 alpha and IL-1 beta selectively decreased the binding of monoclonal antibodies to CD4 on the surface of otherwise unstimulated blood T cells, in contrast to prestimulated and continuously grown CD4+ cells. Under optimal growth conditions, the initial reduction in antibody binding to CD4 was followed by an apparent re-expression of the CD4 antigen even in the presence of high concentrations of IL-1. This re-expression did not occur if the cells were cultured at 4 degrees C, or after treatment with actinomycin D or cytochalasin B, indicating that protein synthesis and intact microfilament function were essential for re-expression of CD4 binding. The mechanism by which CD4 molecules are physically and/or functionally modulated by IL-1 is unclear.

M3 - Journal article

C2 - 3260682

VL - 27

SP - 679

EP - 684

JO - Scandinavian Journal of Immunology

JF - Scandinavian Journal of Immunology

SN - 0300-9475

IS - 6

ER -

ID: 10637698