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Iodination as a probe for small regions of disrupted secondary structure in double-stranded DNA

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Standard

Iodination as a probe for small regions of disrupted secondary structure in double-stranded DNA. / Jensen, Kaj Frank; Nes, Ingolf F.; Wells, Robert D.

I: Nucleic Acids Research, Bind 3, Nr. 11, 1976, s. 3143-3156.

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningfagfællebedømt

Harvard

Jensen, KF, Nes, IF & Wells, RD 1976, 'Iodination as a probe for small regions of disrupted secondary structure in double-stranded DNA', Nucleic Acids Research, bind 3, nr. 11, s. 3143-3156.

APA

Jensen, K. F., Nes, I. F., & Wells, R. D. (1976). Iodination as a probe for small regions of disrupted secondary structure in double-stranded DNA. Nucleic Acids Research, 3(11), 3143-3156.

Vancouver

Jensen KF, Nes IF, Wells RD. Iodination as a probe for small regions of disrupted secondary structure in double-stranded DNA. Nucleic Acids Research. 1976;3(11):3143-3156.

Author

Jensen, Kaj Frank ; Nes, Ingolf F. ; Wells, Robert D. / Iodination as a probe for small regions of disrupted secondary structure in double-stranded DNA. I: Nucleic Acids Research. 1976 ; Bind 3, Nr. 11. s. 3143-3156.

Bibtex

@article{607a5d50847e11dcbee902004c4f4f50,
title = "Iodination as a probe for small regions of disrupted secondary structure in double-stranded DNA",
abstract = "Conditions were established where the thallium-catalyzed iodination of random coil DNA proceeded 100–200 times faster than for native DNA. This reaction was explored as a probe for localized regions of disrupted base pairs in duplex DNA. A heteroduplex was constructed between DNA fragments produced by Hind II + III cleavage of 80 placDNA and 80 plac DNA containing the LI deletion (73 nucleotides in length). This heteroduplex incorporated twelve times as much iodine as the parent homoduplex fragments. Hence the technique could reveal the presence of a few (two or more) nonpaired cytosines, if they existed within an otherwise helical DNA fragment 789 base pairs long. Iodination studies were performed on superhelical SV40 DNA and on linear plac DNA. Analysis of the relative amount of iodine in restriction endonuclease fragments of these DNAs revealed the absence of localized single-stranded regions.",
author = "Jensen, {Kaj Frank} and Nes, {Ingolf F.} and Wells, {Robert D.}",
year = "1976",
language = "English",
volume = "3",
pages = "3143--3156",
journal = "Nucleic Acids Research",
issn = "0305-1048",
publisher = "Oxford University Press",
number = "11",

}

RIS

TY - JOUR

T1 - Iodination as a probe for small regions of disrupted secondary structure in double-stranded DNA

AU - Jensen, Kaj Frank

AU - Nes, Ingolf F.

AU - Wells, Robert D.

PY - 1976

Y1 - 1976

N2 - Conditions were established where the thallium-catalyzed iodination of random coil DNA proceeded 100–200 times faster than for native DNA. This reaction was explored as a probe for localized regions of disrupted base pairs in duplex DNA. A heteroduplex was constructed between DNA fragments produced by Hind II + III cleavage of 80 placDNA and 80 plac DNA containing the LI deletion (73 nucleotides in length). This heteroduplex incorporated twelve times as much iodine as the parent homoduplex fragments. Hence the technique could reveal the presence of a few (two or more) nonpaired cytosines, if they existed within an otherwise helical DNA fragment 789 base pairs long. Iodination studies were performed on superhelical SV40 DNA and on linear plac DNA. Analysis of the relative amount of iodine in restriction endonuclease fragments of these DNAs revealed the absence of localized single-stranded regions.

AB - Conditions were established where the thallium-catalyzed iodination of random coil DNA proceeded 100–200 times faster than for native DNA. This reaction was explored as a probe for localized regions of disrupted base pairs in duplex DNA. A heteroduplex was constructed between DNA fragments produced by Hind II + III cleavage of 80 placDNA and 80 plac DNA containing the LI deletion (73 nucleotides in length). This heteroduplex incorporated twelve times as much iodine as the parent homoduplex fragments. Hence the technique could reveal the presence of a few (two or more) nonpaired cytosines, if they existed within an otherwise helical DNA fragment 789 base pairs long. Iodination studies were performed on superhelical SV40 DNA and on linear plac DNA. Analysis of the relative amount of iodine in restriction endonuclease fragments of these DNAs revealed the absence of localized single-stranded regions.

M3 - Journal article

VL - 3

SP - 3143

EP - 3156

JO - Nucleic Acids Research

JF - Nucleic Acids Research

SN - 0305-1048

IS - 11

ER -

ID: 1393028