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Isolation and life cycle characterization of lytic viruses infecting heterotrophic bacteria and cyanobacteria

Publikation: Bidrag til bog/antologi/rapportBidrag til bog/antologiForskningfagfællebedømt

Basic knowledge on viruses infecting heterotrophic bacteria and cyanobacteria is key to future progress in understanding the role of viruses in aquatic systems and the influence of virus–host interactions on microbial mortality, biogeochemical cycles, and genetic exchange. Such studies require the isolation, propagation, and purification of host–virus systems. This contribution presents some of the most widely used methodological approaches for isolation and purification of bacteriophages and cyanophages, the first step in detailed studies of virus–host interactions and viral genetic composition, and discusses the applications and limitations of different isolation procedures. Most work on phage isolation has been carried out with aerobic heterotrophic bacteria and cyanobacteria, culturable both on agar plates and in enriched liquid cultures. The procedures presented here are limited to lytic viruses infecting such hosts. In addition to the isolation procedures, methods for life cycle characterization (one-step growth experiments) of bacteriophages and cyanophages are described. Finally, limitations and drawbacks of the proposed methods are assessed and discussed
OriginalsprogEngelsk
TitelManual of aquatic viral ecology
RedaktørerS. W. Wilhelm, M. G. Weinbauer, C. A. Suttle
Antal sider16
UdgivelsesstedWaco, Tex
ForlagAmerican Society of Limnology and Oceanography, Inc.
Publikationsdato2010
Sider118-33
Kapitel13
ISBN (Elektronisk)978-0-9845591-0-7
DOI
StatusUdgivet - 2010

ID: 16786724