Forskning ved Københavns Universitet - Københavns Universitet

Forside

Isolation of pyroGlu-Leu-Leu-Gly-Gly-Arg-Phe-NH2 (Pol-RFamide), a novel neuropeptide from hydromedusae

Publikation: Forskning - peer reviewTidsskriftartikel

C J Grimmelikhuijzen, M Hahn, K L Rinehart, A N Spencer

The hydromedusa Polyorchis penicillatus is a good model system to study neurotransmission in coelenterates. Using a radioimmunoassay for the peptide sequence Arg-Phe-NH2 (RFamide), two peptides have now been purified from acetic acid extracts of this medusa. The structure of one of these peptides was established as pyroGlu-Leu-Leu-Gly-Gly-Arg-Phe-NH2, and was named Pol-RFamide. This peptide belongs to the same peptide family as a recently isolated neuropeptide from sea anemones (pyroGlu-Gly-Arg-Phe-NH2). Using antisera to Pol-RFamide, the peptide was found to be exclusively localized in neurones of Polyorchis, among them neurones associated with smooth-muscle fibres. This suggests that Pol-RFamide might be a transmitter or modulator at neuromuscular junctions.
OriginalsprogEngelsk
TidsskriftBrain Research
Vol/bind475
Tidsskriftsnummer1
Sider (fra-til)198-203
Antal sider6
ISSN0006-8993
StatusUdgivet - 13 dec. 1988

ID: 33514388