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Isolation, structural elucidation and in vitro activity of 2-acetyl-2-decarboxamido-oxytetracycline against environmental relevant bacteria, including tetracycline-resistant bacteria

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningfagfællebedømt

Standard

Isolation, structural elucidation and in vitro activity of 2-acetyl-2-decarboxamido-oxytetracycline against environmental relevant bacteria, including tetracycline-resistant bacteria. / Lykkeberg, Anne Kruse; Sengeløv, Gitte; Cornett, Claus; Tjørnelund, Jette; Hansen, Steen Honoré; Halling-Sørensen, Bent.

I: Journal of Pharmaceutical and Biomedical Analysis, Bind 34, Nr. 3, 2004, s. 559-67.

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningfagfællebedømt

Harvard

Lykkeberg, AK, Sengeløv, G, Cornett, C, Tjørnelund, J, Hansen, SH & Halling-Sørensen, B 2004, 'Isolation, structural elucidation and in vitro activity of 2-acetyl-2-decarboxamido-oxytetracycline against environmental relevant bacteria, including tetracycline-resistant bacteria', Journal of Pharmaceutical and Biomedical Analysis, bind 34, nr. 3, s. 559-67.

APA

Lykkeberg, A. K., Sengeløv, G., Cornett, C., Tjørnelund, J., Hansen, S. H., & Halling-Sørensen, B. (2004). Isolation, structural elucidation and in vitro activity of 2-acetyl-2-decarboxamido-oxytetracycline against environmental relevant bacteria, including tetracycline-resistant bacteria. Journal of Pharmaceutical and Biomedical Analysis, 34(3), 559-67.

Vancouver

Lykkeberg AK, Sengeløv G, Cornett C, Tjørnelund J, Hansen SH, Halling-Sørensen B. Isolation, structural elucidation and in vitro activity of 2-acetyl-2-decarboxamido-oxytetracycline against environmental relevant bacteria, including tetracycline-resistant bacteria. Journal of Pharmaceutical and Biomedical Analysis. 2004;34(3):559-67.

Author

Lykkeberg, Anne Kruse ; Sengeløv, Gitte ; Cornett, Claus ; Tjørnelund, Jette ; Hansen, Steen Honoré ; Halling-Sørensen, Bent. / Isolation, structural elucidation and in vitro activity of 2-acetyl-2-decarboxamido-oxytetracycline against environmental relevant bacteria, including tetracycline-resistant bacteria. I: Journal of Pharmaceutical and Biomedical Analysis. 2004 ; Bind 34, Nr. 3. s. 559-67.

Bibtex

@article{e1beb290c69911dd9473000ea68e967b,
title = "Isolation, structural elucidation and in vitro activity of 2-acetyl-2-decarboxamido-oxytetracycline against environmental relevant bacteria, including tetracycline-resistant bacteria",
abstract = "2-Acetyl-2-decarboxamido-oxytetracycline (ADOTC) is a major impurity of oxytetracycline (OTC) produced as a side product during fermentation. ADOTC was isolated from OTC and other impurities using preparative HPLC. The preparative column was an Xterra MS, C18 chromatographic column (100 mm x 19 mm i.d., 5 microm), and the mobile phase contained methanol-water (27:73 (v/v)) with 0.08 M formic acid added. The flow rate was 9.0 ml/min. It was possible to isolate few milligram ADOTC in a day. The compound was unambiguously identified using NMR and MS-MS. The anti-microbial activity against activated sludge bacteria was determined giving a potency of only 3% of that of OTC. With tetracycline-resistant bacteria, no anti-microbial activity was observed, indicating a mode of action similar to that of OTC.",
author = "Lykkeberg, {Anne Kruse} and Gitte Sengel{\o}v and Claus Cornett and Jette Tj{\o}rnelund and Hansen, {Steen Honor{\'e}} and Bent Halling-S{\o}rensen",
note = "Keywords: Animals; Chickens; Chromatography, High Pressure Liquid; Escherichia coli; Microbial Sensitivity Tests; Molecular Structure; Sewage; Swine; Tetracycline Resistance; Tetracyclines",
year = "2004",
language = "English",
volume = "34",
pages = "559--67",
journal = "Journal of Pharmaceutical and Biomedical Analysis",
issn = "0731-7085",
publisher = "Elsevier",
number = "3",

}

RIS

TY - JOUR

T1 - Isolation, structural elucidation and in vitro activity of 2-acetyl-2-decarboxamido-oxytetracycline against environmental relevant bacteria, including tetracycline-resistant bacteria

AU - Lykkeberg, Anne Kruse

AU - Sengeløv, Gitte

AU - Cornett, Claus

AU - Tjørnelund, Jette

AU - Hansen, Steen Honoré

AU - Halling-Sørensen, Bent

N1 - Keywords: Animals; Chickens; Chromatography, High Pressure Liquid; Escherichia coli; Microbial Sensitivity Tests; Molecular Structure; Sewage; Swine; Tetracycline Resistance; Tetracyclines

PY - 2004

Y1 - 2004

N2 - 2-Acetyl-2-decarboxamido-oxytetracycline (ADOTC) is a major impurity of oxytetracycline (OTC) produced as a side product during fermentation. ADOTC was isolated from OTC and other impurities using preparative HPLC. The preparative column was an Xterra MS, C18 chromatographic column (100 mm x 19 mm i.d., 5 microm), and the mobile phase contained methanol-water (27:73 (v/v)) with 0.08 M formic acid added. The flow rate was 9.0 ml/min. It was possible to isolate few milligram ADOTC in a day. The compound was unambiguously identified using NMR and MS-MS. The anti-microbial activity against activated sludge bacteria was determined giving a potency of only 3% of that of OTC. With tetracycline-resistant bacteria, no anti-microbial activity was observed, indicating a mode of action similar to that of OTC.

AB - 2-Acetyl-2-decarboxamido-oxytetracycline (ADOTC) is a major impurity of oxytetracycline (OTC) produced as a side product during fermentation. ADOTC was isolated from OTC and other impurities using preparative HPLC. The preparative column was an Xterra MS, C18 chromatographic column (100 mm x 19 mm i.d., 5 microm), and the mobile phase contained methanol-water (27:73 (v/v)) with 0.08 M formic acid added. The flow rate was 9.0 ml/min. It was possible to isolate few milligram ADOTC in a day. The compound was unambiguously identified using NMR and MS-MS. The anti-microbial activity against activated sludge bacteria was determined giving a potency of only 3% of that of OTC. With tetracycline-resistant bacteria, no anti-microbial activity was observed, indicating a mode of action similar to that of OTC.

M3 - Journal article

C2 - 15127812

VL - 34

SP - 559

EP - 567

JO - Journal of Pharmaceutical and Biomedical Analysis

JF - Journal of Pharmaceutical and Biomedical Analysis

SN - 0731-7085

IS - 3

ER -

ID: 9040296