Forskning ved Københavns Universitet - Københavns Universitet


Laminin from rat yolk sac tumor: isolation, partial characterization, and comparison with mouse laminin

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningfagfællebedømt

Laminin was isolated from a rat yolk sac tumor by salt extraction, gel filtration, and affinity chromatography on heparin-Sepharose. The purified laminin gave two polypeptide chains with approximate Mr of 200,000 and 400,000 in sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Its amino acid composition and electron microscopic appearance were similar to those reported earlier for mouse laminin. Carbohydrate analysis revealed 13% carbohydrate consisting of N-acetylglucosamine, galactose, mannose, fucose, sialic acid, and small amounts of N-acetyl galactosamine. The purified rat laminin was immunologically very similar to mouse laminin as recognized by rabbit antibodies but was antigenically distinct when recognized by mouse antibodies.
TidsskriftArchives of Biochemistry and Biophysics
Udgave nummer2
Sider (fra-til)649-56
Antal sider8
StatusUdgivet - 15 apr. 1983

ID: 34330412