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Lck is involved in interleukin-2 induced proliferation but not cell survival in human T cells through a MAP kinase-independent pathway

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Standard

Lck is involved in interleukin-2 induced proliferation but not cell survival in human T cells through a MAP kinase-independent pathway. / Brockdorff, J; Nielsen, M; Kaltoft, K; Mustelin, T; Röpke, C; Svejaard, A; Geisler, C; Odum, N.

I: European Cytokine Network, Bind 11, Nr. 2, 2000, s. 225-31.

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningfagfællebedømt

Harvard

Brockdorff, J, Nielsen, M, Kaltoft, K, Mustelin, T, Röpke, C, Svejaard, A, Geisler, C & Odum, N 2000, 'Lck is involved in interleukin-2 induced proliferation but not cell survival in human T cells through a MAP kinase-independent pathway', European Cytokine Network, bind 11, nr. 2, s. 225-31.

APA

Brockdorff, J., Nielsen, M., Kaltoft, K., Mustelin, T., Röpke, C., Svejaard, A., ... Odum, N. (2000). Lck is involved in interleukin-2 induced proliferation but not cell survival in human T cells through a MAP kinase-independent pathway. European Cytokine Network, 11(2), 225-31.

Vancouver

Brockdorff J, Nielsen M, Kaltoft K, Mustelin T, Röpke C, Svejaard A o.a. Lck is involved in interleukin-2 induced proliferation but not cell survival in human T cells through a MAP kinase-independent pathway. European Cytokine Network. 2000;11(2):225-31.

Author

Brockdorff, J ; Nielsen, M ; Kaltoft, K ; Mustelin, T ; Röpke, C ; Svejaard, A ; Geisler, C ; Odum, N. / Lck is involved in interleukin-2 induced proliferation but not cell survival in human T cells through a MAP kinase-independent pathway. I: European Cytokine Network. 2000 ; Bind 11, Nr. 2. s. 225-31.

Bibtex

@article{77edb300b0a211ddb538000ea68e967b,
title = "Lck is involved in interleukin-2 induced proliferation but not cell survival in human T cells through a MAP kinase-independent pathway",
abstract = "The role of Lck in IL-2-induced proliferation and cell survival is still controversial. Here, we show that the Src family kinase inhibitor, PP1, reduced the IL-2-induced proliferation of human T cells significantly without inhibiting the anti-apoptotic effect of IL-2. As Lck is the only Src family kinase activated upon IL-2 stimulation in T cells, this indicates that Lck is involved in IL-2-induced proliferation but not survival. IL-2-induced MAP kinase activation was only slightly inhibited by PP1, suggesting that Lck is not essential for IL-2-induced MAP kinase activation in human T cells. We found that an IL-2-sensitive, human mycosis fungoides-derived tumor T cell line is Lck negative, and that the IL-2-induced MAP kinase activation is comparable to non-cancerous T cells, although a little delayed in kinetics. An Lck expressing clone was established by transfecting Lck into mycosis fungoides tumor T cells, but Lck had no influence on the delayed kinetics of MAP kinase activation, indicating that Lck is not essential for MAP kinase activation in mycosis fungoides tumor T cells or in non-cancerous T cells. Taken together, this indicates that Lck is involved in IL-2-induced proliferation, but not cell survival, through a pathway not involving MAP kinase.",
author = "J Brockdorff and M Nielsen and K Kaltoft and T Mustelin and C R{\"o}pke and A Svejaard and C Geisler and N Odum",
note = "Keywords: Base Sequence; Cell Division; Cell Line; Cell Survival; DNA Primers; Enzyme Activation; Enzyme Inhibitors; Humans; Interleukin-2; Lymphocyte Specific Protein Tyrosine Kinase p56(lck); Mitogen-Activated Protein Kinases; Mycosis Fungoides; Pyrazoles; Pyrimidines; RNA, Neoplasm; T-Lymphocytes; Tumor Cells, Cultured",
year = "2000",
language = "English",
volume = "11",
pages = "225--31",
journal = "European Cytokine Network (Online)",
issn = "1148-5493",
publisher = "JohnLibbey Eurotext",
number = "2",

}

RIS

TY - JOUR

T1 - Lck is involved in interleukin-2 induced proliferation but not cell survival in human T cells through a MAP kinase-independent pathway

AU - Brockdorff, J

AU - Nielsen, M

AU - Kaltoft, K

AU - Mustelin, T

AU - Röpke, C

AU - Svejaard, A

AU - Geisler, C

AU - Odum, N

N1 - Keywords: Base Sequence; Cell Division; Cell Line; Cell Survival; DNA Primers; Enzyme Activation; Enzyme Inhibitors; Humans; Interleukin-2; Lymphocyte Specific Protein Tyrosine Kinase p56(lck); Mitogen-Activated Protein Kinases; Mycosis Fungoides; Pyrazoles; Pyrimidines; RNA, Neoplasm; T-Lymphocytes; Tumor Cells, Cultured

PY - 2000

Y1 - 2000

N2 - The role of Lck in IL-2-induced proliferation and cell survival is still controversial. Here, we show that the Src family kinase inhibitor, PP1, reduced the IL-2-induced proliferation of human T cells significantly without inhibiting the anti-apoptotic effect of IL-2. As Lck is the only Src family kinase activated upon IL-2 stimulation in T cells, this indicates that Lck is involved in IL-2-induced proliferation but not survival. IL-2-induced MAP kinase activation was only slightly inhibited by PP1, suggesting that Lck is not essential for IL-2-induced MAP kinase activation in human T cells. We found that an IL-2-sensitive, human mycosis fungoides-derived tumor T cell line is Lck negative, and that the IL-2-induced MAP kinase activation is comparable to non-cancerous T cells, although a little delayed in kinetics. An Lck expressing clone was established by transfecting Lck into mycosis fungoides tumor T cells, but Lck had no influence on the delayed kinetics of MAP kinase activation, indicating that Lck is not essential for MAP kinase activation in mycosis fungoides tumor T cells or in non-cancerous T cells. Taken together, this indicates that Lck is involved in IL-2-induced proliferation, but not cell survival, through a pathway not involving MAP kinase.

AB - The role of Lck in IL-2-induced proliferation and cell survival is still controversial. Here, we show that the Src family kinase inhibitor, PP1, reduced the IL-2-induced proliferation of human T cells significantly without inhibiting the anti-apoptotic effect of IL-2. As Lck is the only Src family kinase activated upon IL-2 stimulation in T cells, this indicates that Lck is involved in IL-2-induced proliferation but not survival. IL-2-induced MAP kinase activation was only slightly inhibited by PP1, suggesting that Lck is not essential for IL-2-induced MAP kinase activation in human T cells. We found that an IL-2-sensitive, human mycosis fungoides-derived tumor T cell line is Lck negative, and that the IL-2-induced MAP kinase activation is comparable to non-cancerous T cells, although a little delayed in kinetics. An Lck expressing clone was established by transfecting Lck into mycosis fungoides tumor T cells, but Lck had no influence on the delayed kinetics of MAP kinase activation, indicating that Lck is not essential for MAP kinase activation in mycosis fungoides tumor T cells or in non-cancerous T cells. Taken together, this indicates that Lck is involved in IL-2-induced proliferation, but not cell survival, through a pathway not involving MAP kinase.

M3 - Journal article

C2 - 10903801

VL - 11

SP - 225

EP - 231

JO - European Cytokine Network (Online)

JF - European Cytokine Network (Online)

SN - 1148-5493

IS - 2

ER -

ID: 8545021