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Liquid-phase microextraction of drugs from human breast milk

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Standard

Liquid-phase microextraction of drugs from human breast milk. / Bjørhovde, Anett; Halvorsen, Trine Grønhaug; Rasmussen, Knut Einar; Pedersen-Bjergaard, Stig.

I: Analytica Chimica Acta, Bind 491, Nr. 2, 08.09.2003, s. 155-161.

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningfagfællebedømt

Harvard

Bjørhovde, A, Halvorsen, TG, Rasmussen, KE & Pedersen-Bjergaard, S 2003, 'Liquid-phase microextraction of drugs from human breast milk', Analytica Chimica Acta, bind 491, nr. 2, s. 155-161. https://doi.org/10.1016/S0003-2670(03)00768-2

APA

Bjørhovde, A., Halvorsen, T. G., Rasmussen, K. E., & Pedersen-Bjergaard, S. (2003). Liquid-phase microextraction of drugs from human breast milk. Analytica Chimica Acta, 491(2), 155-161. https://doi.org/10.1016/S0003-2670(03)00768-2

Vancouver

Bjørhovde A, Halvorsen TG, Rasmussen KE, Pedersen-Bjergaard S. Liquid-phase microextraction of drugs from human breast milk. Analytica Chimica Acta. 2003 sep 8;491(2):155-161. https://doi.org/10.1016/S0003-2670(03)00768-2

Author

Bjørhovde, Anett ; Halvorsen, Trine Grønhaug ; Rasmussen, Knut Einar ; Pedersen-Bjergaard, Stig. / Liquid-phase microextraction of drugs from human breast milk. I: Analytica Chimica Acta. 2003 ; Bind 491, Nr. 2. s. 155-161.

Bibtex

@article{7cb7d91572f34d80bc6b38437b6fa613,
title = "Liquid-phase microextraction of drugs from human breast milk",
abstract = "Liquid-phase microextraction (LPME) based on disposable porous polypropylene hollow fibers was evaluated for the extraction of hydrophobic basic drugs from human breast milk. Direct LPME from breast milk samples provided low recoveries (18-38%) because the drugs were partially bound to the sample matrix (fat). Therefore, prior to extraction, the breast milk was acidified and the majority of fat was removed by centrifugation. The pH-reduction in the breast milk was performed to release drugs interacting with the matrix. From the supernatant, where pH was adjusted into the alkaline region with NaOH to deionize the analytes, the drugs were extracted through a thin layer of polyphenyl-methylsiloxane present in the pores of a porous hollow fiber and into 15μl of 10mM HCl as acceptor solution present inside the lumen of the hollow fiber. Subsequently, the acceptor solution was directly subjected to capillary electrophoresis (CE). For four antidepressant drugs, recoveries in the range 42-69% were obtained from breast milk, the drugs were enriched by a factor of 14-23 during LPME, excellent sample clean-up was observed, linearity (r>0.99) was obtained in the range 50-500ng/ml, and the extractions were found to be independent of the fat content in the breast milk samples.",
keywords = "Antidepressant drugs, Breast milk, Capillary electrophoresis, Hollow fibers, Liquid-phase microextraction",
author = "Anett Bj{\o}rhovde and Halvorsen, {Trine Gr{\o}nhaug} and Rasmussen, {Knut Einar} and Stig Pedersen-Bjergaard",
year = "2003",
month = sep,
day = "8",
doi = "10.1016/S0003-2670(03)00768-2",
language = "English",
volume = "491",
pages = "155--161",
journal = "Analytica Chimica Acta",
issn = "0003-2670",
publisher = "Elsevier",
number = "2",

}

RIS

TY - JOUR

T1 - Liquid-phase microextraction of drugs from human breast milk

AU - Bjørhovde, Anett

AU - Halvorsen, Trine Grønhaug

AU - Rasmussen, Knut Einar

AU - Pedersen-Bjergaard, Stig

PY - 2003/9/8

Y1 - 2003/9/8

N2 - Liquid-phase microextraction (LPME) based on disposable porous polypropylene hollow fibers was evaluated for the extraction of hydrophobic basic drugs from human breast milk. Direct LPME from breast milk samples provided low recoveries (18-38%) because the drugs were partially bound to the sample matrix (fat). Therefore, prior to extraction, the breast milk was acidified and the majority of fat was removed by centrifugation. The pH-reduction in the breast milk was performed to release drugs interacting with the matrix. From the supernatant, where pH was adjusted into the alkaline region with NaOH to deionize the analytes, the drugs were extracted through a thin layer of polyphenyl-methylsiloxane present in the pores of a porous hollow fiber and into 15μl of 10mM HCl as acceptor solution present inside the lumen of the hollow fiber. Subsequently, the acceptor solution was directly subjected to capillary electrophoresis (CE). For four antidepressant drugs, recoveries in the range 42-69% were obtained from breast milk, the drugs were enriched by a factor of 14-23 during LPME, excellent sample clean-up was observed, linearity (r>0.99) was obtained in the range 50-500ng/ml, and the extractions were found to be independent of the fat content in the breast milk samples.

AB - Liquid-phase microextraction (LPME) based on disposable porous polypropylene hollow fibers was evaluated for the extraction of hydrophobic basic drugs from human breast milk. Direct LPME from breast milk samples provided low recoveries (18-38%) because the drugs were partially bound to the sample matrix (fat). Therefore, prior to extraction, the breast milk was acidified and the majority of fat was removed by centrifugation. The pH-reduction in the breast milk was performed to release drugs interacting with the matrix. From the supernatant, where pH was adjusted into the alkaline region with NaOH to deionize the analytes, the drugs were extracted through a thin layer of polyphenyl-methylsiloxane present in the pores of a porous hollow fiber and into 15μl of 10mM HCl as acceptor solution present inside the lumen of the hollow fiber. Subsequently, the acceptor solution was directly subjected to capillary electrophoresis (CE). For four antidepressant drugs, recoveries in the range 42-69% were obtained from breast milk, the drugs were enriched by a factor of 14-23 during LPME, excellent sample clean-up was observed, linearity (r>0.99) was obtained in the range 50-500ng/ml, and the extractions were found to be independent of the fat content in the breast milk samples.

KW - Antidepressant drugs

KW - Breast milk

KW - Capillary electrophoresis

KW - Hollow fibers

KW - Liquid-phase microextraction

UR - http://www.scopus.com/inward/record.url?scp=0142091508&partnerID=8YFLogxK

U2 - 10.1016/S0003-2670(03)00768-2

DO - 10.1016/S0003-2670(03)00768-2

M3 - Journal article

AN - SCOPUS:0142091508

VL - 491

SP - 155

EP - 161

JO - Analytica Chimica Acta

JF - Analytica Chimica Acta

SN - 0003-2670

IS - 2

ER -

ID: 231652437