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L-leucine methyl ester stimulates insulin secretion and islet glutamate dehydrogenase

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Standard

L-leucine methyl ester stimulates insulin secretion and islet glutamate dehydrogenase. / Knudsen, P; Kofod, Hans; Lernmark, A; Hedeskov, C J.

I: American Journal of Physiology (Consolidated), Bind 245, Nr. 4, 10.1983, s. E338-46.

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningfagfællebedømt

Harvard

Knudsen, P, Kofod, H, Lernmark, A & Hedeskov, CJ 1983, 'L-leucine methyl ester stimulates insulin secretion and islet glutamate dehydrogenase', American Journal of Physiology (Consolidated), bind 245, nr. 4, s. E338-46.

APA

Knudsen, P., Kofod, H., Lernmark, A., & Hedeskov, C. J. (1983). L-leucine methyl ester stimulates insulin secretion and islet glutamate dehydrogenase. American Journal of Physiology (Consolidated), 245(4), E338-46.

Vancouver

Knudsen P, Kofod H, Lernmark A, Hedeskov CJ. L-leucine methyl ester stimulates insulin secretion and islet glutamate dehydrogenase. American Journal of Physiology (Consolidated). 1983 okt;245(4):E338-46.

Author

Knudsen, P ; Kofod, Hans ; Lernmark, A ; Hedeskov, C J. / L-leucine methyl ester stimulates insulin secretion and islet glutamate dehydrogenase. I: American Journal of Physiology (Consolidated). 1983 ; Bind 245, Nr. 4. s. E338-46.

Bibtex

@article{676a51f765aa4f89969b86bfd2eaf081,
title = "L-leucine methyl ester stimulates insulin secretion and islet glutamate dehydrogenase",
abstract = "Column perifusion of collagenase-isolated mouse pancreatic islets was used to study the dynamics of insulin release in experiments lasting for several hours. The methyl esters of L-leucine and L-arginine were synthesized. Whereas L-arginine methyl ester (L-arginine OMe) had no effect, L-leucine OMe stimulated the release of insulin. The effect of L-leucine OMe was maximal at 5 mmol/liter. Whereas the Km for glucose-stimulated insulin release was unaffected by 1 mmol/liter L-leucine OMe, the maximal release of D-glucose was increased by the amino acid derivative that appeared more effective than L-leucine. L-Leucine OMe was also a potent stimulus of insulin release from the perfused mouse pancreas. In the presence of 10 mmol/liter L-glutamine, 1 mmol/liter L-leucine OMe induced a 50- to 75-fold increase in insulin release. A similar stimulatory effect was also observed in column-perifused RIN 5F cells, a cloned rat islet tumor cell line. A twofold increase in islet glutamate dehydrogenase activity was induced by 5 mmol/liter L-leucine OMe, a larger effect than that of L-leucine (P less than 0.02), whereas L-arginine OMe had a small inhibitory effect. We conclude that L-leucine OMe is a potent stimulus of insulin secretion and that its effect on the beta-cells may be exerted by activating islet glutamate dehydrogenase.",
keywords = "1-Methyl-3-isobutylxanthine, Animals, Cell Line, Glucagon, Glucose, Glutamate Dehydrogenase, Glutamine, Insulin, Insulinoma, Islets of Langerhans, Kinetics, Leucine, Male, Mice, Mice, Inbred Strains, Norepinephrine, Pancreatic Neoplasms, Rats",
author = "P Knudsen and Hans Kofod and A Lernmark and Hedeskov, {C J}",
year = "1983",
month = "10",
language = "English",
volume = "245",
pages = "E338--46",
journal = "American Journal of Physiology (Consolidated)",
issn = "0002-9513",
publisher = "American Physiological Society",
number = "4",

}

RIS

TY - JOUR

T1 - L-leucine methyl ester stimulates insulin secretion and islet glutamate dehydrogenase

AU - Knudsen, P

AU - Kofod, Hans

AU - Lernmark, A

AU - Hedeskov, C J

PY - 1983/10

Y1 - 1983/10

N2 - Column perifusion of collagenase-isolated mouse pancreatic islets was used to study the dynamics of insulin release in experiments lasting for several hours. The methyl esters of L-leucine and L-arginine were synthesized. Whereas L-arginine methyl ester (L-arginine OMe) had no effect, L-leucine OMe stimulated the release of insulin. The effect of L-leucine OMe was maximal at 5 mmol/liter. Whereas the Km for glucose-stimulated insulin release was unaffected by 1 mmol/liter L-leucine OMe, the maximal release of D-glucose was increased by the amino acid derivative that appeared more effective than L-leucine. L-Leucine OMe was also a potent stimulus of insulin release from the perfused mouse pancreas. In the presence of 10 mmol/liter L-glutamine, 1 mmol/liter L-leucine OMe induced a 50- to 75-fold increase in insulin release. A similar stimulatory effect was also observed in column-perifused RIN 5F cells, a cloned rat islet tumor cell line. A twofold increase in islet glutamate dehydrogenase activity was induced by 5 mmol/liter L-leucine OMe, a larger effect than that of L-leucine (P less than 0.02), whereas L-arginine OMe had a small inhibitory effect. We conclude that L-leucine OMe is a potent stimulus of insulin secretion and that its effect on the beta-cells may be exerted by activating islet glutamate dehydrogenase.

AB - Column perifusion of collagenase-isolated mouse pancreatic islets was used to study the dynamics of insulin release in experiments lasting for several hours. The methyl esters of L-leucine and L-arginine were synthesized. Whereas L-arginine methyl ester (L-arginine OMe) had no effect, L-leucine OMe stimulated the release of insulin. The effect of L-leucine OMe was maximal at 5 mmol/liter. Whereas the Km for glucose-stimulated insulin release was unaffected by 1 mmol/liter L-leucine OMe, the maximal release of D-glucose was increased by the amino acid derivative that appeared more effective than L-leucine. L-Leucine OMe was also a potent stimulus of insulin release from the perfused mouse pancreas. In the presence of 10 mmol/liter L-glutamine, 1 mmol/liter L-leucine OMe induced a 50- to 75-fold increase in insulin release. A similar stimulatory effect was also observed in column-perifused RIN 5F cells, a cloned rat islet tumor cell line. A twofold increase in islet glutamate dehydrogenase activity was induced by 5 mmol/liter L-leucine OMe, a larger effect than that of L-leucine (P less than 0.02), whereas L-arginine OMe had a small inhibitory effect. We conclude that L-leucine OMe is a potent stimulus of insulin secretion and that its effect on the beta-cells may be exerted by activating islet glutamate dehydrogenase.

KW - 1-Methyl-3-isobutylxanthine

KW - Animals

KW - Cell Line

KW - Glucagon

KW - Glucose

KW - Glutamate Dehydrogenase

KW - Glutamine

KW - Insulin

KW - Insulinoma

KW - Islets of Langerhans

KW - Kinetics

KW - Leucine

KW - Male

KW - Mice

KW - Mice, Inbred Strains

KW - Norepinephrine

KW - Pancreatic Neoplasms

KW - Rats

M3 - Journal article

C2 - 6194691

VL - 245

SP - E338-46

JO - American Journal of Physiology (Consolidated)

JF - American Journal of Physiology (Consolidated)

SN - 0002-9513

IS - 4

ER -

ID: 45575352