Forskning ved Københavns Universitet - Københavns Universitet


L-Type calcium channels but not N-methyl-D-aspartate receptor channels mediate rhythmic activity induced by cholinergic agonist in motoneurons from turtle spinal cord slices

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningfagfællebedømt

Rhythmic activity induced by different combinations of N-methyl-D- aspartate (NMDA), serotonin (5-HT), muscarine and D-tubocurarine was monitored intracellularly in lumbar motoneurons in a slice preparation from adult turtles. Low concentration of NMDA (7.5-15 μM) combined with 5-HT (10- 80 μM) induced rhythmic motoneuron discharge which was undefiled by intrinsic voltage oscillations resistant to tetrodotoxin. This oscillatory activity was abolished by 2-amino-5-phosphonopentanoic acid (AP5), a competitive blocker of NMDA receptors and by nifedipine a selective blocker of L-type calcium channels. In contrast, rhythmicity induced by the cholinergic agents muscarine and d-tubocurarine was abolished by nifedipine but not by AP5 nor by high [Mg2+]0. These results show that different receptor agonists induce intrinsic oscillations in mature motoneurons by independent routes. Each oscillatory mechanism depends on I-type calcium channels but only NMDA/5-HT-induced oscillations depend on voltage-sensitive NMDA-activated ionophores.

TidsskriftNeuroscience Letters
Udgave nummer1-2
Sider (fra-til)81-84
Antal sider4
StatusUdgivet - 12 feb. 1999

ID: 237699073