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Mathematical modeling and statistical analysis of calcium-regulated insulin granule exocytosis in ß-cells from mice and humans

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  • Morten Gram Pedersen
  • Giuliana Cortese
  • Lena Eliasson
Insulin is released from pancreatic ß-cells as a result of Ca2+-evoked exocytosis of dense-core granules. Secretion is biphasic, which has been suggested to correspond to the release of different granule pools. Here we review and carefully reanalyze previously published patch-clamp data on depolarization-evoked Ca2+-currents and corresponding capacitance measurements. Using a statistical mixed-effects model, we show that the data indicate that pool depletion is negligible in response to short depolarizations in mouse ß-cells. We then review mathematical models of granule dynamics and exocytosis in rodent ß-cells and present a mathematical description of Ca2+-evoked exocytosis in human ß-cells, which show clear differences to their rodent counterparts. The model suggests that L- and P/Q-type Ca2+-channels are involved to a similar degree in exocytosis during electrical activity in human ß-cells.

OriginalsprogEngelsk
TidsskriftProgress in Biophysics & Molecular Biology
Vol/bind107
Udgave nummer2
Sider (fra-til)257-264
ISSN0079-6107
DOI
StatusUdgivet - 2011

ID: 38345266