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Maturation of human dendritic cells by monocyte-conditioned medium is dependent upon trace amounts of lipopolysaccharide inducing tumour necrosis factor alpha

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  • Jacob Nersting
  • Morten Svenson
  • Vagn Andersen
  • Klaus Bendtzen
We investigated the ability of monocyte-conditioned medium (MCM), generated by monocytes cultured on plastic-immobilised immunoglobulin, to stimulate maturation of human monocyte-derived dendritic cells (DC). Earlier reports suggest that MCM is a strong inducer of irreversible DC maturation, whereas we find, that adding a small amount of lipopolysaccharide (LPS) to the MCM-generating cultures is required for the production of a DC-stimulatory MCM. Moreover, compared with addition of LPS directly to the DC cultures, stimulation via MCM cultures increases by several fold the DC-stimulatory potency of LPS. Maturation by this procedure is mediated mainly by tumour necrosis factor alpha secreted from monocytes during the medium-conditioning period.
OriginalsprogEngelsk
TidsskriftImmunology Letters
Vol/bind89
Udgave nummer1
Sider (fra-til)59-65
Antal sider7
ISSN0165-2478
StatusUdgivet - 9 okt. 2003

ID: 34102033