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Melon ascorbate oxidase: Cloning of a multigene family, induction during fruit development and repression by wounding

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningfagfællebedømt

  • George Diallinas
  • Pateraki, Irini
  • Maite Sanmartin
  • Angela Scossa
  • Eugenia Stilianou
  • Nickolas J. Panopoulos
  • Angelos K. Kanellis

A small family of at least four genes encoding melon ascorbate oxidase (AO) has been identified and three members of it have been cloned. Preliminary DNA sequence determination suggested that melon AO genes code for enzymes homologous to ascorbate oxidases from other plants and similar to other multicopper oxidases. We describe detailed molecular studies addressing melon AO expression during organ specific differentiation, fruit development and ripening, and in response to wounding. In particular, AO transcript accumulation was induced in ovaries and the outer mesocarp of mature preclimacteric melon fruits, before the expression of genes encoding the necessary enzymatic activities for ethylene biosynthesis. On the other hand, AO was not expressed in late stages of fruit ripening and was repressed in wounded fruits. The role of ethylene in transcriptional regulation of AO is discussed.

OriginalsprogEngelsk
TidsskriftPlant Molecular Biology
Vol/bind34
Udgave nummer5
Sider (fra-til)759-770
Antal sider12
ISSN0167-4412
DOI
StatusUdgivet - 1 jul. 1997
Eksternt udgivetJa

ID: 131465854