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Mesitylene-Cored Glucoside Amphiphiles (MGAs) for Membrane Protein Studies: Importance of Alkyl Chain Density in Detergent Efficacy

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Standard

Mesitylene-Cored Glucoside Amphiphiles (MGAs) for Membrane Protein Studies: Importance of Alkyl Chain Density in Detergent Efficacy. / Cho, Kyung Ho; Ribeiro, Orquidea; Du, Yang; Tikhonova, Elena; Mortensen, Jonas; Markham, Kelsey; Hariharan, Parameswaran; Løland, Claus; Guan, Lan; Kobilka, Brian; Byrne, Bernadette; Chae, Pil Seok.

I: Chemistry: A European Journal, Bind 22, Nr. 52, 23.12.2016, s. 18833–18839.

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningfagfællebedømt

Harvard

Cho, KH, Ribeiro, O, Du, Y, Tikhonova, E, Mortensen, J, Markham, K, Hariharan, P, Løland, C, Guan, L, Kobilka, B, Byrne, B & Chae, PS 2016, 'Mesitylene-Cored Glucoside Amphiphiles (MGAs) for Membrane Protein Studies: Importance of Alkyl Chain Density in Detergent Efficacy', Chemistry: A European Journal, bind 22, nr. 52, s. 18833–18839. https://doi.org/10.1002/chem.201603338

APA

Cho, K. H., Ribeiro, O., Du, Y., Tikhonova, E., Mortensen, J., Markham, K., ... Chae, P. S. (2016). Mesitylene-Cored Glucoside Amphiphiles (MGAs) for Membrane Protein Studies: Importance of Alkyl Chain Density in Detergent Efficacy. Chemistry: A European Journal, 22(52), 18833–18839. https://doi.org/10.1002/chem.201603338

Vancouver

Cho KH, Ribeiro O, Du Y, Tikhonova E, Mortensen J, Markham K o.a. Mesitylene-Cored Glucoside Amphiphiles (MGAs) for Membrane Protein Studies: Importance of Alkyl Chain Density in Detergent Efficacy. Chemistry: A European Journal. 2016 dec 23;22(52):18833–18839. https://doi.org/10.1002/chem.201603338

Author

Cho, Kyung Ho ; Ribeiro, Orquidea ; Du, Yang ; Tikhonova, Elena ; Mortensen, Jonas ; Markham, Kelsey ; Hariharan, Parameswaran ; Løland, Claus ; Guan, Lan ; Kobilka, Brian ; Byrne, Bernadette ; Chae, Pil Seok. / Mesitylene-Cored Glucoside Amphiphiles (MGAs) for Membrane Protein Studies: Importance of Alkyl Chain Density in Detergent Efficacy. I: Chemistry: A European Journal. 2016 ; Bind 22, Nr. 52. s. 18833–18839.

Bibtex

@article{4144a44644bb435fa639374bab5d8dfc,
title = "Mesitylene-Cored Glucoside Amphiphiles (MGAs) for Membrane Protein Studies: Importance of Alkyl Chain Density in Detergent Efficacy",
abstract = "Detergents serve as useful tools for membrane protein structural and functional studies. Their amphipathic nature allows detergents to associate with the hydrophobic regions of membrane proteins whilst maintaining the proteins in aqueous solution. However, widely used conventional detergents are limited in their ability to maintain the structural integrity of membrane proteins and thus there are major efforts underway to develop novel agents with improved properties. We prepared mesitylene-cored glucoside amphiphiles (MGAs) with three alkyl chains and compared these agents with previously developed xylene-linked maltoside agents (XMAs) with two alkyl chains and a conventional detergent (DDM). When these agents were evaluated for four membrane proteins including a G protein-coupled receptor (GPCR), some agents such as MGA-C13 and MGA-C14 resulted in markedly enhanced stability of membrane proteins compared to both DDM and the XMAs. This favourable behaviour is due likely to the increased hydrophobic density provided by the extra alkyl chain. Thus, this study not only describes new glucoside agents with potential for membrane protein research, but also introduces a new detergent design principle for future development.",
author = "Cho, {Kyung Ho} and Orquidea Ribeiro and Yang Du and Elena Tikhonova and Jonas Mortensen and Kelsey Markham and Parameswaran Hariharan and Claus L{\o}land and Lan Guan and Brian Kobilka and Bernadette Byrne and Chae, {Pil Seok}",
note = "{\circledC} 2016 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.",
year = "2016",
month = "12",
day = "23",
doi = "10.1002/chem.201603338",
language = "English",
volume = "22",
pages = "18833–18839",
journal = "Chemistry: A European Journal",
issn = "0947-6539",
publisher = "Wiley - V C H Verlag GmbH & Co. KGaA",
number = "52",

}

RIS

TY - JOUR

T1 - Mesitylene-Cored Glucoside Amphiphiles (MGAs) for Membrane Protein Studies: Importance of Alkyl Chain Density in Detergent Efficacy

AU - Cho, Kyung Ho

AU - Ribeiro, Orquidea

AU - Du, Yang

AU - Tikhonova, Elena

AU - Mortensen, Jonas

AU - Markham, Kelsey

AU - Hariharan, Parameswaran

AU - Løland, Claus

AU - Guan, Lan

AU - Kobilka, Brian

AU - Byrne, Bernadette

AU - Chae, Pil Seok

N1 - © 2016 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

PY - 2016/12/23

Y1 - 2016/12/23

N2 - Detergents serve as useful tools for membrane protein structural and functional studies. Their amphipathic nature allows detergents to associate with the hydrophobic regions of membrane proteins whilst maintaining the proteins in aqueous solution. However, widely used conventional detergents are limited in their ability to maintain the structural integrity of membrane proteins and thus there are major efforts underway to develop novel agents with improved properties. We prepared mesitylene-cored glucoside amphiphiles (MGAs) with three alkyl chains and compared these agents with previously developed xylene-linked maltoside agents (XMAs) with two alkyl chains and a conventional detergent (DDM). When these agents were evaluated for four membrane proteins including a G protein-coupled receptor (GPCR), some agents such as MGA-C13 and MGA-C14 resulted in markedly enhanced stability of membrane proteins compared to both DDM and the XMAs. This favourable behaviour is due likely to the increased hydrophobic density provided by the extra alkyl chain. Thus, this study not only describes new glucoside agents with potential for membrane protein research, but also introduces a new detergent design principle for future development.

AB - Detergents serve as useful tools for membrane protein structural and functional studies. Their amphipathic nature allows detergents to associate with the hydrophobic regions of membrane proteins whilst maintaining the proteins in aqueous solution. However, widely used conventional detergents are limited in their ability to maintain the structural integrity of membrane proteins and thus there are major efforts underway to develop novel agents with improved properties. We prepared mesitylene-cored glucoside amphiphiles (MGAs) with three alkyl chains and compared these agents with previously developed xylene-linked maltoside agents (XMAs) with two alkyl chains and a conventional detergent (DDM). When these agents were evaluated for four membrane proteins including a G protein-coupled receptor (GPCR), some agents such as MGA-C13 and MGA-C14 resulted in markedly enhanced stability of membrane proteins compared to both DDM and the XMAs. This favourable behaviour is due likely to the increased hydrophobic density provided by the extra alkyl chain. Thus, this study not only describes new glucoside agents with potential for membrane protein research, but also introduces a new detergent design principle for future development.

U2 - 10.1002/chem.201603338

DO - 10.1002/chem.201603338

M3 - Journal article

C2 - 27743406

VL - 22

SP - 18833

EP - 18839

JO - Chemistry: A European Journal

JF - Chemistry: A European Journal

SN - 0947-6539

IS - 52

ER -

ID: 167933153