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Metabolism of prostaglandin E and of glutathione conjugate of prostaglandin A (GSH-prostaglandin A) by prostaglandin 9-ketoreductase from rabbit kidney

Publikation: Forskning - peer reviewTidsskriftartikel

B.S. Toft, Harald S. Hansen

Rabbit kidney prostaglandin 9-ketoreductase was found to metabolize the glutathione conjugate of prostaglandin A (GSH-prostaglandin A). Apparent K (GSH-prostaglandin A) 13 µM and apparent K (prostaglandin E) 200 µM. The cytosolic preparation was subjected to gelfiltration and isoelectric focusing, which revealed that metabolism of prostaglandin E and GSH-prostaglandin A occurs by means of the same fractions. Furthermore, prostaglandin E and GSH-prostaglandin A are competitive inhibitors of the enzyme, when GSH-prostaglandin A and prostaglandin E are tested as substrates, respectively. It is concluded, that GSH-prostaglandin A is a much better substrate for prostaglandin 9-ketoreductase from rabbit kidney than is prostaglandin E.
OriginalsprogEngelsk
TidsskriftB B A - Molecular and Cell Biology of Lipids
Vol/bind574
Tidsskriftsnummer1
Sider (fra-til)33-38
Antal sider6
ISSN1388-1981
StatusUdgivet - 27 jul. 1979

ID: 45562933