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Modifications of cholera toxin subunit B binding to human large intestinal epithelium: an immunohistochemical study

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Modifications of cholera toxin subunit B binding to human large intestinal epithelium : an immunohistochemical study. / Kirkeby, Svend; Pedersen, Anne Marie Lynge.

I: Microbial Pathogenesis, Bind 124, 2018, s. 332-336.

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningfagfællebedømt

Harvard

Kirkeby, S & Pedersen, AML 2018, 'Modifications of cholera toxin subunit B binding to human large intestinal epithelium: an immunohistochemical study', Microbial Pathogenesis, bind 124, s. 332-336. https://doi.org/10.1016/j.micpath.2018.08.047

APA

Kirkeby, S., & Pedersen, A. M. L. (2018). Modifications of cholera toxin subunit B binding to human large intestinal epithelium: an immunohistochemical study. Microbial Pathogenesis, 124, 332-336. https://doi.org/10.1016/j.micpath.2018.08.047

Vancouver

Kirkeby S, Pedersen AML. Modifications of cholera toxin subunit B binding to human large intestinal epithelium: an immunohistochemical study. Microbial Pathogenesis. 2018;124:332-336. https://doi.org/10.1016/j.micpath.2018.08.047

Author

Kirkeby, Svend ; Pedersen, Anne Marie Lynge. / Modifications of cholera toxin subunit B binding to human large intestinal epithelium : an immunohistochemical study. I: Microbial Pathogenesis. 2018 ; Bind 124. s. 332-336.

Bibtex

@article{6cffa9585b224606ac5f52d871e39718,
title = "Modifications of cholera toxin subunit B binding to human large intestinal epithelium: an immunohistochemical study",
abstract = "Binding of cholera toxin subunit B (CTB) to its receptor and toxin transport into the intestinal epithelial cells are the causative events for the potentially lethal disease cholera. The five sugar mono-sialo ganglioside GM1 is the cell surface receptor for cholera toxin B-subunit. CTB binding was determined by use of immobilized GM1 to microtiter plates and by immunohistochemistry. Sections from the human colon and the human soft palate were incubated with FITC-conjugated CTB and with anti-MUC2. Both the luminal surface of the intestine and the secretory goblet cells exhibited strong binding. Addition of simple carbohydrates and milk to the incubation medium showed that a combination of lactose and non-fat dry milk was potent inhibitors of toxin- and mucin binding. Both CTB and ant-MUC2 stained to the cytoplasm (mucin granules) in the goblet cells from the human soft palate. In the colon CTB stained the entire cytoplasm of the goblet cells while anti-MUC2 detected only the supranuclear region of some cells, suggesting carbohydrate heterogeneity between goblet cell mucin granules in different regions of the human body. Both CTB- and MUC2 binding were inhibited when GM1 was added to the incubation medium. It is proposed that the human colonic goblet cells play a role in the secretory diarrhea in patients with cholera and that milk might have a prophylactic or therapeutic application in the management of cholera.",
author = "Svend Kirkeby and Pedersen, {Anne Marie Lynge}",
note = "Copyright {\circledC} 2018. Published by Elsevier Ltd.",
year = "2018",
doi = "10.1016/j.micpath.2018.08.047",
language = "English",
volume = "124",
pages = "332--336",
journal = "Microbial Pathogenesis",
issn = "0882-4010",
publisher = "Academic Press",

}

RIS

TY - JOUR

T1 - Modifications of cholera toxin subunit B binding to human large intestinal epithelium

T2 - an immunohistochemical study

AU - Kirkeby, Svend

AU - Pedersen, Anne Marie Lynge

N1 - Copyright © 2018. Published by Elsevier Ltd.

PY - 2018

Y1 - 2018

N2 - Binding of cholera toxin subunit B (CTB) to its receptor and toxin transport into the intestinal epithelial cells are the causative events for the potentially lethal disease cholera. The five sugar mono-sialo ganglioside GM1 is the cell surface receptor for cholera toxin B-subunit. CTB binding was determined by use of immobilized GM1 to microtiter plates and by immunohistochemistry. Sections from the human colon and the human soft palate were incubated with FITC-conjugated CTB and with anti-MUC2. Both the luminal surface of the intestine and the secretory goblet cells exhibited strong binding. Addition of simple carbohydrates and milk to the incubation medium showed that a combination of lactose and non-fat dry milk was potent inhibitors of toxin- and mucin binding. Both CTB and ant-MUC2 stained to the cytoplasm (mucin granules) in the goblet cells from the human soft palate. In the colon CTB stained the entire cytoplasm of the goblet cells while anti-MUC2 detected only the supranuclear region of some cells, suggesting carbohydrate heterogeneity between goblet cell mucin granules in different regions of the human body. Both CTB- and MUC2 binding were inhibited when GM1 was added to the incubation medium. It is proposed that the human colonic goblet cells play a role in the secretory diarrhea in patients with cholera and that milk might have a prophylactic or therapeutic application in the management of cholera.

AB - Binding of cholera toxin subunit B (CTB) to its receptor and toxin transport into the intestinal epithelial cells are the causative events for the potentially lethal disease cholera. The five sugar mono-sialo ganglioside GM1 is the cell surface receptor for cholera toxin B-subunit. CTB binding was determined by use of immobilized GM1 to microtiter plates and by immunohistochemistry. Sections from the human colon and the human soft palate were incubated with FITC-conjugated CTB and with anti-MUC2. Both the luminal surface of the intestine and the secretory goblet cells exhibited strong binding. Addition of simple carbohydrates and milk to the incubation medium showed that a combination of lactose and non-fat dry milk was potent inhibitors of toxin- and mucin binding. Both CTB and ant-MUC2 stained to the cytoplasm (mucin granules) in the goblet cells from the human soft palate. In the colon CTB stained the entire cytoplasm of the goblet cells while anti-MUC2 detected only the supranuclear region of some cells, suggesting carbohydrate heterogeneity between goblet cell mucin granules in different regions of the human body. Both CTB- and MUC2 binding were inhibited when GM1 was added to the incubation medium. It is proposed that the human colonic goblet cells play a role in the secretory diarrhea in patients with cholera and that milk might have a prophylactic or therapeutic application in the management of cholera.

U2 - 10.1016/j.micpath.2018.08.047

DO - 10.1016/j.micpath.2018.08.047

M3 - Journal article

C2 - 30145256

VL - 124

SP - 332

EP - 336

JO - Microbial Pathogenesis

JF - Microbial Pathogenesis

SN - 0882-4010

ER -

ID: 201807812