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Molecular cloning of a novel, putative G protein-coupled receptor from sea anemones structurally related to members of the FSH, TSH, LH/CG receptor family from mammals

Publikation: Forskning - peer reviewTidsskriftartikel

H P Nothacker, C J Grimmelikhuijzen

Using oligonucleotide probes derived from consensus sequences of known vertebrate and invertebrate G protein-coupled receptors, we have cloned the cDNA for a presumed G protein-coupled receptor from sea anemones. This receptor shows a striking structural homology with members of the glycoprotein hormone (FSH, TSH, LH/CG) receptor family from mammals, including a very large, extracellular N terminus (18-25% sequence identity) and a 7 transmembrane region (44-48% sequence identity). As with the mammalian glycoprotein hormone receptor genes, the sea anemone receptor gene yields transcripts which can be alternatively spliced, thereby yielding a shortened receptor variant only containing the large extracellular (soluble) N terminus. All this is strong evidence that the putative glycoprotein hormone receptor from sea anemones is evolutionarily related to those from mammals. This is the first report showing that a putative glycoprotein hormone receptor occurs in invertebrates.
OriginalsprogEngelsk
TidsskriftBiochemical and Biophysical Research Communications
Vol/bind197
Tidsskriftsnummer3
Sider (fra-til)1062-9
Antal sider8
ISSN0006-291X
DOI
StatusUdgivet - 30 dec. 1993

ID: 33513847