Forskning ved Københavns Universitet - Københavns Universitet


MT1-MMP-dependent, apoptotic remodeling of unmineralized cartilage: a critical process in skeletal growth

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  • Holmbeck, Kenn
  • Paolo Bianco
  • Kali Chrysovergis
  • Susan Yamada
  • Henning Birkedal-Hansen

Skeletal tissues develop either by intramembranous ossification, where bone is formed within a soft connective tissue, or by endochondral ossification. The latter proceeds via cartilage anlagen, which through hypertrophy, mineralization, and partial resorption ultimately provides scaffolding for bone formation. Here, we describe a novel and essential mechanism governing remodeling of unmineralized cartilage anlagen into membranous bone, as well as tendons and ligaments. Membrane-type 1 matrix metalloproteinase (MT1-MMP)-dependent dissolution of unmineralized cartilages, coupled with apoptosis of nonhypertrophic chondrocytes, mediates remodeling of these cartilages into other tissues. The MT1-MMP deficiency disrupts this process and uncouples apoptotic demise of chondrocytes and cartilage degradation, resulting in the persistence of "ghost" cartilages with adverse effects on skeletal integrity. Some cells entrapped in these ghost cartilages escape apoptosis, maintain DNA synthesis, and assume phenotypes normally found in the tissues replacing unmineralized cartilages. The coordinated apoptosis and matrix metalloproteinase-directed cartilage dissolution is akin to metamorphosis and may thus represent its evolutionary legacy in mammals.

TidsskriftThe Journal of Cell Biology
Udgave nummer3
Sider (fra-til)661-71
Antal sider11
StatusUdgivet - 10 nov. 2003

ID: 201164544