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Mutations in cyr1 and pat1 reveal pheromone-induced G1 arrest in the fission yeast Schizosaccharomyces pombe

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Investigations into sexual differentiation and pheromone response in the fission yeast Schizosaccharomyces pombe are complicated by the need to first starve the cells of nitrogen. Most mating-related experiments are therefore performed on non-dividing cells. Here we overcome this problem by using two mutants that bypass the nutritional requirements and respond to the M-factor mating pheromone in rich medium. The first mutant lacks the cyr1 gene which encodes adenylate cyclase and these cells contain no measurable amounts of cAMP. When M-factor is added to a growing h+ cyr1- strain it causes a transient G1 arrest of cell division, transcription of mat1-Pm, and elongation of the cells to form shmoos. The second mutant contains the temperature-sensitive pat1-114 allele. At 30 degrees C this mutant was previously shown not only to bypass the nutritional signal but also to stop growing in a state derepressed for pheromone-controlled functions. We now report that an h+ pat1-114 strain growing mitotically at 23 degrees C responds to M-factor. This shows that the pat1 protein kinase can be tuned to derepress nutritional signalling while repressing the other stages in the differentiation process.
OriginalsprogEngelsk
TidsskriftCurrent Genetics
Vol/bind26
Udgave nummer2
Sider (fra-til)105-12
Antal sider8
ISSN0172-8083
StatusUdgivet - 1 aug. 1994

ID: 33577371