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NanoSIMS Analysis of Intravascular Lipolysis and Lipid Movement across Capillaries and into Cardiomyocytes

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  • nihms957147

    Accepteret manuskript, 3,28 MB, PDF-dokument

  • Cuiwen He
  • Thomas A Weston
  • Rachel S Jung
  • Patrick Heizer
  • Mikael Larsson
  • Xuchen Hu
  • Christopher M Allan
  • Peter Tontonoz
  • Karen Reue
  • Anne P Beigneux
  • Ploug, Michael
  • Andrea Holme
  • Matthew Kilburn
  • Paul Guagliardo
  • David A Ford
  • Loren G Fong
  • Stephen G Young
  • Haibo Jiang

The processing of triglyceride-rich lipoproteins (TRLs) in capillaries provides lipids for vital tissues, but our understanding of TRL metabolism is limited, in part because TRL processing and lipid movement have never been visualized. To investigate the movement of TRL-derived lipids in the heart, mice were given an injection of [2H]triglyceride-enriched TRLs, and the movement of 2H-labeled lipids across capillaries and into cardiomyocytes was examined by NanoSIMS. TRL processing and lipid movement in tissues were extremely rapid. Within 30 s, TRL-derived lipids appeared in the subendothelial spaces and in the lipid droplets and mitochondria of cardiomyocytes. Enrichment of 2H in capillary endothelial cells was not greater than in cardiomyocytes, implying that endothelial cells may not be a control point for lipid movement into cardiomyocytes. Remarkably, a deficiency of the putative fatty acid transport protein CD36, which is expressed highly in capillary endothelial cells, did not impede entry of TRL-derived lipids into cardiomyocytes.

OriginalsprogEngelsk
TidsskriftCell Metabolism
Vol/bind27
Udgave nummer5
Sider (fra-til)1055-1066.e3
Antal sider16
ISSN1550-4131
DOI
StatusUdgivet - 1 maj 2018

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