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Non-core Subunits of the PRC2 Complex Are Collectively Required for Its Target-Site Specificity

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Non-core Subunits of the PRC2 Complex Are Collectively Required for Its Target-Site Specificity. / Højfeldt, Jonas Westergaard; Hedehus, Lin; Laugesen, Anne; Tatar, Tülin; Wiehle, Laura; Helin, Kristian.

I: Molecular Cell, Bind 76, Nr. 3, E3, 07.11.2019, s. 423-436.

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningfagfællebedømt

Harvard

Højfeldt, JW, Hedehus, L, Laugesen, A, Tatar, T, Wiehle, L & Helin, K 2019, 'Non-core Subunits of the PRC2 Complex Are Collectively Required for Its Target-Site Specificity', Molecular Cell, bind 76, nr. 3, E3, s. 423-436. https://doi.org/10.1016/j.molcel.2019.07.031

APA

Højfeldt, J. W., Hedehus, L., Laugesen, A., Tatar, T., Wiehle, L., & Helin, K. (2019). Non-core Subunits of the PRC2 Complex Are Collectively Required for Its Target-Site Specificity. Molecular Cell, 76(3), 423-436. [E3]. https://doi.org/10.1016/j.molcel.2019.07.031

Vancouver

Højfeldt JW, Hedehus L, Laugesen A, Tatar T, Wiehle L, Helin K. Non-core Subunits of the PRC2 Complex Are Collectively Required for Its Target-Site Specificity. Molecular Cell. 2019 nov 7;76(3):423-436. E3. https://doi.org/10.1016/j.molcel.2019.07.031

Author

Højfeldt, Jonas Westergaard ; Hedehus, Lin ; Laugesen, Anne ; Tatar, Tülin ; Wiehle, Laura ; Helin, Kristian. / Non-core Subunits of the PRC2 Complex Are Collectively Required for Its Target-Site Specificity. I: Molecular Cell. 2019 ; Bind 76, Nr. 3. s. 423-436.

Bibtex

@article{8b00da50e5664a478bebd1bd11666555,
title = "Non-core Subunits of the PRC2 Complex Are Collectively Required for Its Target-Site Specificity",
abstract = "The Polycomb repressive complex 2 (PRC2) catalyzes H3K27 methylation across the genome, which impacts transcriptional regulation and is critical for establishment of cell identity. Because of its essential function during development and in cancer, understanding the delineation of genome-wide H3K27 methylation patterns has been the focus of intense investigation. PRC2 methylation activity is abundant and dispersed throughout the genome, but the highest activity is specifically directed to a subset of target sites that are stably occupied by the complex and highly enriched for H3K27me3. Here, we show, by systematically knocking out single and multiple non-core subunits of the PRC2 complex in mouse embryonic stem cells, that they each contribute to directing PRC2 activity to target sites. Furthermore, combined knockout of six non-core subunits reveals that, while dispensable for global H3K27 methylation levels, the non-core PRC2 subunits are collectively required for focusing H3K27me3 activity to specific sites in the genome.",
author = "H{\o}jfeldt, {Jonas Westergaard} and Lin Hedehus and Anne Laugesen and T{\"u}lin Tatar and Laura Wiehle and Kristian Helin",
note = "Copyright {\circledC} 2019 Elsevier Inc. All rights reserved.",
year = "2019",
month = "11",
day = "7",
doi = "10.1016/j.molcel.2019.07.031",
language = "English",
volume = "76",
pages = "423--436",
journal = "Molecular Cell",
issn = "1097-2765",
publisher = "Cell Press",
number = "3",

}

RIS

TY - JOUR

T1 - Non-core Subunits of the PRC2 Complex Are Collectively Required for Its Target-Site Specificity

AU - Højfeldt, Jonas Westergaard

AU - Hedehus, Lin

AU - Laugesen, Anne

AU - Tatar, Tülin

AU - Wiehle, Laura

AU - Helin, Kristian

N1 - Copyright © 2019 Elsevier Inc. All rights reserved.

PY - 2019/11/7

Y1 - 2019/11/7

N2 - The Polycomb repressive complex 2 (PRC2) catalyzes H3K27 methylation across the genome, which impacts transcriptional regulation and is critical for establishment of cell identity. Because of its essential function during development and in cancer, understanding the delineation of genome-wide H3K27 methylation patterns has been the focus of intense investigation. PRC2 methylation activity is abundant and dispersed throughout the genome, but the highest activity is specifically directed to a subset of target sites that are stably occupied by the complex and highly enriched for H3K27me3. Here, we show, by systematically knocking out single and multiple non-core subunits of the PRC2 complex in mouse embryonic stem cells, that they each contribute to directing PRC2 activity to target sites. Furthermore, combined knockout of six non-core subunits reveals that, while dispensable for global H3K27 methylation levels, the non-core PRC2 subunits are collectively required for focusing H3K27me3 activity to specific sites in the genome.

AB - The Polycomb repressive complex 2 (PRC2) catalyzes H3K27 methylation across the genome, which impacts transcriptional regulation and is critical for establishment of cell identity. Because of its essential function during development and in cancer, understanding the delineation of genome-wide H3K27 methylation patterns has been the focus of intense investigation. PRC2 methylation activity is abundant and dispersed throughout the genome, but the highest activity is specifically directed to a subset of target sites that are stably occupied by the complex and highly enriched for H3K27me3. Here, we show, by systematically knocking out single and multiple non-core subunits of the PRC2 complex in mouse embryonic stem cells, that they each contribute to directing PRC2 activity to target sites. Furthermore, combined knockout of six non-core subunits reveals that, while dispensable for global H3K27 methylation levels, the non-core PRC2 subunits are collectively required for focusing H3K27me3 activity to specific sites in the genome.

U2 - 10.1016/j.molcel.2019.07.031

DO - 10.1016/j.molcel.2019.07.031

M3 - Journal article

C2 - 31521506

VL - 76

SP - 423

EP - 436

JO - Molecular Cell

JF - Molecular Cell

SN - 1097-2765

IS - 3

M1 - E3

ER -

ID: 227413285