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Nucleotide sequences of cDNA clones for C-hordein polypeptides

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  • Søren K. Rasmussen
  • Anders Brandt

Two partial cDNA clones, pc hor1-3 and pc-919, encoding the carboxyterminal portion of two different C-hordein polypeptides have been sequenced. pc hor1-3 can be translated into a frame of 74 amino acids, part of which is identical to the amino acid sequence of a C-hordein chymotryptic peptide. The 3′ non-coding region is 174 nucleotides in length and extends to the polyadenylation site. The region has two putative polyadenylation signals, AATAAA, located 27 and 80 nucleotides upstream from the poly(A)-tail. Three RNA species, 1800, 2100 and 4300 bases in length, can be detected in the endosperm using pc hor1-3 as probe. These could code for C-hordein polypeptides of 48, 67 and 144 kD, respectively. Hybridization of pc hor1-3 to restriction endonuclease fragments of nuclear barley DNA suggests that 6-8 genes code for the C-hordein polypeptides. The nucleotide sequence of the second cDNA clone, pc-919, can be translated into 64 amino acids, of which the 22 carboxyterminal amino acid residues are identical to the carboxyterminal reading frame encoded by pc hor1-3. The adjacent 42 amino acid long reading frame shows homology to that deduced from pc hor1-3, but the nucleotide sequence is different and highly homologous to the 5′ coding sequence found in a B-hordein genomic clone. Hybridization using this part of pc-919 as a probe detects RNAs of 1200 and 1400 bases only. Thus pc-919 represents a gene containing sequences related to both Hor1 and the Hor2 locus, and is the first nucleotide sequence data giving evidence that the two loci are related.

TidsskriftCarlsberg Research Communications
Udgave nummer6
Sider (fra-til)371-379
Antal sider9
StatusUdgivet - 1 jan. 1986

ID: 204471750