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OPEN PULLED STRAW (OPS) VITRIFICATION OF PORCINE BLASTOCYSTS: SIMPLE PROCEDURE YIELDING EXCELLENT IN VITRO SURVIVAL, BUT SO FAR NO PIGLETS FOLLOWING TRANSFER

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  • Holm, Peter
  • G Vajta
  • Z Macháty
  • M Schmidt
  • R.S. Prather
  • T Greve
  • H Callesen
In 2 trials, 346 porcine in vivo blastocysts were vitrified in heat-softened and pulled French straws by the Open Pulled Straw (OPS) vitrification method. After thawing, embryos were either cultured in vitro (n=89) for 24 to 48 hours or transferred surgically (n=257) to 11 synchronised recipients without further culture. In trial I. 88% and 71 % of thawed blastocysts reexpanded and hatched in vitro, respectively, while 71 % reexpanded in trial 2. However, no piglets were born following transfer. though 1 recipient was diagnosed pregnant at Day 56 of gestation.
OriginalsprogEngelsk
TidsskriftCryo-Letters
Udgave nummer20
Sider (fra-til)307-310
Antal sider4
ISSN0143-2044
StatusUdgivet - 1999

    Forskningsområder

  • cryopreservation,embryo survival,embryos,swine

ID: 141568461