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Peptide nucleic acid: a versatile tool in genetic diagnostics and molecular biology

Publikation: Bidrag til tidsskriftReviewForskning

During the past ten years, the DNA mimic peptide nucleic acid has inspired the development of a variety of hybridisation-based methods for detection, quantification, purification and characterisation of nucleic acids. Most of these methods have taken advantage of the very favourable DNA and RNA hybridisation properties of peptide nucleic acids combined with the unique properties and opportunities offered by peptide chemistry. Within the past year, significant progress in in situ hybridisation technology has been achieved, which has resulted, in particular, in reliable and sensitive methods for detection of bacteria in clinical samples, as well as in environmental samples. Furthermore, applications of the polymerase chain reaction clamping method have been expanded, and novel ways of exploiting complexes of peptide nucleic acids with double-stranded DNA, such as double duplex invasion complexes and PD loops, have been developed.

OriginalsprogEngelsk
TidsskriftCurrent Opinion in Biotechnology
Vol/bind12
Udgave nummer1
Sider (fra-til)16-20
Antal sider5
ISSN0958-1669
StatusUdgivet - feb. 2001

ID: 203635660