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Peptide nucleic acid (PNA) antisense effects in Escherichia coli

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Standard

Peptide nucleic acid (PNA) antisense effects in Escherichia coli. / Good, L; Nielsen, P E.

I: Current Issues in Molecular Biology, Bind 1, Nr. 1-2, 1999, s. 111-6.

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningfagfællebedømt

Harvard

Good, L & Nielsen, PE 1999, 'Peptide nucleic acid (PNA) antisense effects in Escherichia coli', Current Issues in Molecular Biology, bind 1, nr. 1-2, s. 111-6.

APA

Good, L., & Nielsen, P. E. (1999). Peptide nucleic acid (PNA) antisense effects in Escherichia coli. Current Issues in Molecular Biology, 1(1-2), 111-6.

Vancouver

Good L, Nielsen PE. Peptide nucleic acid (PNA) antisense effects in Escherichia coli. Current Issues in Molecular Biology. 1999;1(1-2):111-6.

Author

Good, L ; Nielsen, P E. / Peptide nucleic acid (PNA) antisense effects in Escherichia coli. I: Current Issues in Molecular Biology. 1999 ; Bind 1, Nr. 1-2. s. 111-6.

Bibtex

@article{b502cbb4fda24dcbb4a60a6b5ef70d99,
title = "Peptide nucleic acid (PNA) antisense effects in Escherichia coli",
abstract = "Antisense peptide nucleic acid (PNA) can be used to control cell growth, gene expression and growth phenotypes in the bacteria Escherichia coli. PNAs targeted to the RNA components of the ribosome can inhibit translation and cell growth, and PNAs targeted to mRNA can limit gene expression with gene and sequence specificity. In an E. coli cell extract, efficient inhibition is observed when using PNA concentrations in the nanomolar range, whereas micromolar concentrations are required for inhibition in growing cells. A mutant strain of E. coli that is more permeable to antibiotics also is more susceptible to antisense PNAs than the wild type. This chapter details methods for testing the antisense activities of PNA in E. coli. As an example of the specific antisense inhibition possible, we show the effects of an anti-beta-galactosidase PNA in comparison to control PNAs. With improvements in cell uptake, antisense PNAs may find applications as antimicrobial agents and as tools for microbial functional genomics.",
keywords = "Antisense Elements (Genetics), Escherichia coli, Gene Expression Regulation, Peptide Nucleic Acids, beta-Galactosidase",
author = "L Good and Nielsen, {P E}",
year = "1999",
language = "English",
volume = "1",
pages = "111--6",
journal = "Current Issues in Molecular Biology",
issn = "1467-3037",
publisher = "Caister Academic Press",
number = "1-2",

}

RIS

TY - JOUR

T1 - Peptide nucleic acid (PNA) antisense effects in Escherichia coli

AU - Good, L

AU - Nielsen, P E

PY - 1999

Y1 - 1999

N2 - Antisense peptide nucleic acid (PNA) can be used to control cell growth, gene expression and growth phenotypes in the bacteria Escherichia coli. PNAs targeted to the RNA components of the ribosome can inhibit translation and cell growth, and PNAs targeted to mRNA can limit gene expression with gene and sequence specificity. In an E. coli cell extract, efficient inhibition is observed when using PNA concentrations in the nanomolar range, whereas micromolar concentrations are required for inhibition in growing cells. A mutant strain of E. coli that is more permeable to antibiotics also is more susceptible to antisense PNAs than the wild type. This chapter details methods for testing the antisense activities of PNA in E. coli. As an example of the specific antisense inhibition possible, we show the effects of an anti-beta-galactosidase PNA in comparison to control PNAs. With improvements in cell uptake, antisense PNAs may find applications as antimicrobial agents and as tools for microbial functional genomics.

AB - Antisense peptide nucleic acid (PNA) can be used to control cell growth, gene expression and growth phenotypes in the bacteria Escherichia coli. PNAs targeted to the RNA components of the ribosome can inhibit translation and cell growth, and PNAs targeted to mRNA can limit gene expression with gene and sequence specificity. In an E. coli cell extract, efficient inhibition is observed when using PNA concentrations in the nanomolar range, whereas micromolar concentrations are required for inhibition in growing cells. A mutant strain of E. coli that is more permeable to antibiotics also is more susceptible to antisense PNAs than the wild type. This chapter details methods for testing the antisense activities of PNA in E. coli. As an example of the specific antisense inhibition possible, we show the effects of an anti-beta-galactosidase PNA in comparison to control PNAs. With improvements in cell uptake, antisense PNAs may find applications as antimicrobial agents and as tools for microbial functional genomics.

KW - Antisense Elements (Genetics)

KW - Escherichia coli

KW - Gene Expression Regulation

KW - Peptide Nucleic Acids

KW - beta-Galactosidase

UR - http://www.horizonpress.com/cimb/v/v1/111.pdf

M3 - Journal article

C2 - 11475695

VL - 1

SP - 111

EP - 116

JO - Current Issues in Molecular Biology

JF - Current Issues in Molecular Biology

SN - 1467-3037

IS - 1-2

ER -

ID: 154515272