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Performance of four different indirect enzyme-linked immunosorbent assays (ELISAs) to detect specific IgG, IgA, and IgM in Legionnaires' disease

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Standard

Performance of four different indirect enzyme-linked immunosorbent assays (ELISAs) to detect specific IgG, IgA, and IgM in Legionnaires' disease. / Bangsborg, Jette Marie; Shand, G H; Hansen, K; Wright, Erin J.

I: A P M I S. Acta Pathologica, Microbiologica et Immunologica Scandinavica, Bind 102, Nr. 7, 1994, s. 501-8.

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningfagfællebedømt

Harvard

Bangsborg, JM, Shand, GH, Hansen, K & Wright, EJ 1994, 'Performance of four different indirect enzyme-linked immunosorbent assays (ELISAs) to detect specific IgG, IgA, and IgM in Legionnaires' disease', A P M I S. Acta Pathologica, Microbiologica et Immunologica Scandinavica, bind 102, nr. 7, s. 501-8.

APA

Bangsborg, J. M., Shand, G. H., Hansen, K., & Wright, E. J. (1994). Performance of four different indirect enzyme-linked immunosorbent assays (ELISAs) to detect specific IgG, IgA, and IgM in Legionnaires' disease. A P M I S. Acta Pathologica, Microbiologica et Immunologica Scandinavica, 102(7), 501-8.

Vancouver

Bangsborg JM, Shand GH, Hansen K, Wright EJ. Performance of four different indirect enzyme-linked immunosorbent assays (ELISAs) to detect specific IgG, IgA, and IgM in Legionnaires' disease. A P M I S. Acta Pathologica, Microbiologica et Immunologica Scandinavica. 1994;102(7):501-8.

Author

Bangsborg, Jette Marie ; Shand, G H ; Hansen, K ; Wright, Erin J. / Performance of four different indirect enzyme-linked immunosorbent assays (ELISAs) to detect specific IgG, IgA, and IgM in Legionnaires' disease. I: A P M I S. Acta Pathologica, Microbiologica et Immunologica Scandinavica. 1994 ; Bind 102, Nr. 7. s. 501-8.

Bibtex

@article{11e2f5332b364d21a3e043949a44f0a0,
title = "Performance of four different indirect enzyme-linked immunosorbent assays (ELISAs) to detect specific IgG, IgA, and IgM in Legionnaires' disease",
abstract = "Currently recommended methods in Legionnaires' disease serology are based upon crude whole-cell antigenic preparations. To investigate whether purified antigens would perform better in a given diagnostic test for antibodies against Legionella pneumophila, we compared the performance of three antigenic preparations of L. pneumophila serogroup 1 consisting of outer membrane protein (OMP), flagellin (FLA), and lipopolysaccharide (LPS) to a sonic extract (SON) in indirect immunosorbent assay (ELISA) measuring both IgG, IgA, and IgM. The reactivity of sera from 20 patients with culture-verified Legionnaires' disease and sera from 12 patients with pneumonia and a diagnostic rise in titre by a microagglutination test (MA) was studied. Our results indicated that the SON IgA assay was the most sensitive test in both groups of patients. The LPS IgG and IgM assays, however, were the most specific tests, closely followed by the corresponding SON tests. By combining two individual assays, a maximum nosographic sensitivity of 85% could be obtained. Whereas no benefit of using purified outer membrane protein or flagella instead of a sonic extract in the indirect ELISAs was found, the LPS antigen provided a sensitive and specific alternative to the sonic extract.",
keywords = "Antibodies, Bacterial, Bacterial Outer Membrane Proteins, Enzyme-Linked Immunosorbent Assay, Flagellin, Humans, Immunoglobulin A, Immunoglobulin G, Immunoglobulin M, Immunoglobulins, Legionella pneumophila, Legionnaires' Disease, Lipopolysaccharides, Sensitivity and Specificity",
author = "Bangsborg, {Jette Marie} and Shand, {G H} and K Hansen and Wright, {Erin J.}",
year = "1994",
language = "English",
volume = "102",
pages = "501--8",
journal = "A P M I S. Acta Pathologica, Microbiologica et Immunologica Scandinavica",
issn = "0903-4641",
publisher = "Wiley Online",
number = "7",

}

RIS

TY - JOUR

T1 - Performance of four different indirect enzyme-linked immunosorbent assays (ELISAs) to detect specific IgG, IgA, and IgM in Legionnaires' disease

AU - Bangsborg, Jette Marie

AU - Shand, G H

AU - Hansen, K

AU - Wright, Erin J.

PY - 1994

Y1 - 1994

N2 - Currently recommended methods in Legionnaires' disease serology are based upon crude whole-cell antigenic preparations. To investigate whether purified antigens would perform better in a given diagnostic test for antibodies against Legionella pneumophila, we compared the performance of three antigenic preparations of L. pneumophila serogroup 1 consisting of outer membrane protein (OMP), flagellin (FLA), and lipopolysaccharide (LPS) to a sonic extract (SON) in indirect immunosorbent assay (ELISA) measuring both IgG, IgA, and IgM. The reactivity of sera from 20 patients with culture-verified Legionnaires' disease and sera from 12 patients with pneumonia and a diagnostic rise in titre by a microagglutination test (MA) was studied. Our results indicated that the SON IgA assay was the most sensitive test in both groups of patients. The LPS IgG and IgM assays, however, were the most specific tests, closely followed by the corresponding SON tests. By combining two individual assays, a maximum nosographic sensitivity of 85% could be obtained. Whereas no benefit of using purified outer membrane protein or flagella instead of a sonic extract in the indirect ELISAs was found, the LPS antigen provided a sensitive and specific alternative to the sonic extract.

AB - Currently recommended methods in Legionnaires' disease serology are based upon crude whole-cell antigenic preparations. To investigate whether purified antigens would perform better in a given diagnostic test for antibodies against Legionella pneumophila, we compared the performance of three antigenic preparations of L. pneumophila serogroup 1 consisting of outer membrane protein (OMP), flagellin (FLA), and lipopolysaccharide (LPS) to a sonic extract (SON) in indirect immunosorbent assay (ELISA) measuring both IgG, IgA, and IgM. The reactivity of sera from 20 patients with culture-verified Legionnaires' disease and sera from 12 patients with pneumonia and a diagnostic rise in titre by a microagglutination test (MA) was studied. Our results indicated that the SON IgA assay was the most sensitive test in both groups of patients. The LPS IgG and IgM assays, however, were the most specific tests, closely followed by the corresponding SON tests. By combining two individual assays, a maximum nosographic sensitivity of 85% could be obtained. Whereas no benefit of using purified outer membrane protein or flagella instead of a sonic extract in the indirect ELISAs was found, the LPS antigen provided a sensitive and specific alternative to the sonic extract.

KW - Antibodies, Bacterial

KW - Bacterial Outer Membrane Proteins

KW - Enzyme-Linked Immunosorbent Assay

KW - Flagellin

KW - Humans

KW - Immunoglobulin A

KW - Immunoglobulin G

KW - Immunoglobulin M

KW - Immunoglobulins

KW - Legionella pneumophila

KW - Legionnaires' Disease

KW - Lipopolysaccharides

KW - Sensitivity and Specificity

M3 - Journal article

C2 - 7917219

VL - 102

SP - 501

EP - 508

JO - A P M I S. Acta Pathologica, Microbiologica et Immunologica Scandinavica

JF - A P M I S. Acta Pathologica, Microbiologica et Immunologica Scandinavica

SN - 0903-4641

IS - 7

ER -

ID: 40333983