Forskning ved Københavns Universitet - Københavns Universitet


Phosphorylation networks regulating JNK activity in diverse genetic backgrounds

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningfagfællebedømt

  • Chris Bakal
  • Rune Linding
  • Flora Llense
  • Elleard Heffern
  • Enrique Martin-Blanco
  • Tony Pawson
  • Norbert Perrimon

Cellular signaling networks have evolved to enable swift and accurate responses, even in the face of genetic or environmental perturbation. Thus, genetic screens may not identify all the genes that regulate different biological processes. Moreover, although classical screening approaches have succeeded in providing parts lists of the essential components of signaling networks, they typically do not provide much insight into the hierarchical and functional relations that exist among these components. We describe a high-throughput screen in which we used RNA interference to systematically inhibit two genes simultaneously in 17,724 combinations to identify regulators of Drosophila JUN NH(2)-terminal kinase (JNK). Using both genetic and phosphoproteomics data, we then implemented an integrative network algorithm to construct a JNK phosphorylation network, which provides structural and mechanistic insights into the systems architecture of JNK signaling.

TidsskriftScience (New York, N.Y.)
Udgave nummer5900
Sider (fra-til)453-456
Antal sider4
StatusUdgivet - 17 okt. 2008
Eksternt udgivetJa

ID: 122677774