Forskning ved Københavns Universitet - Københavns Universitet

Forside

Photocleavage of DNA and photofootprinting of E. coli RNA polymerase bound to promoter DNA by azido-9-acridinylamines

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningfagfællebedømt

Standard

Photocleavage of DNA and photofootprinting of E. coli RNA polymerase bound to promoter DNA by azido-9-acridinylamines. / Buchardt, O; Henriksen, U; Nielsen, Peter E.

I: Nucleic Acids Research, Bind 16, Nr. 13, 11.07.1988, s. 5755-70.

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningfagfællebedømt

Harvard

Buchardt, O, Henriksen, U & Nielsen, PE 1988, 'Photocleavage of DNA and photofootprinting of E. coli RNA polymerase bound to promoter DNA by azido-9-acridinylamines', Nucleic Acids Research, bind 16, nr. 13, s. 5755-70.

APA

Buchardt, O., Henriksen, U., & Nielsen, P. E. (1988). Photocleavage of DNA and photofootprinting of E. coli RNA polymerase bound to promoter DNA by azido-9-acridinylamines. Nucleic Acids Research, 16(13), 5755-70.

Vancouver

Buchardt O, Henriksen U, Nielsen PE. Photocleavage of DNA and photofootprinting of E. coli RNA polymerase bound to promoter DNA by azido-9-acridinylamines. Nucleic Acids Research. 1988 jul 11;16(13):5755-70.

Author

Buchardt, O ; Henriksen, U ; Nielsen, Peter E. / Photocleavage of DNA and photofootprinting of E. coli RNA polymerase bound to promoter DNA by azido-9-acridinylamines. I: Nucleic Acids Research. 1988 ; Bind 16, Nr. 13. s. 5755-70.

Bibtex

@article{ba96312e4e7e481ab3d1af80c3019240,
title = "Photocleavage of DNA and photofootprinting of E. coli RNA polymerase bound to promoter DNA by azido-9-acridinylamines",
abstract = "The long-wavelength ultraviolet (lambda approximately 420 nm) radiation induced reaction between 6-azido-2-methoxy-9-acridinylamines and supercoiled plasmid DNA results in single strand scissions and formation of covalent adducts (ratio approximately 1:10). By treating azidoacridine-photomodified DNA with piperidine at 90 degrees C, additional strand scissions are observed in a complex sequence dependent manner with an overall preference for T greater than or equal to G greater than C much greater than A. The resulting DNA fragments migrate as 5'-phosphates in polyacrylamide gels. Photofootprinting of the binding site of RNA-polymerase on promoter DNA is demonstrated with an azido-9-acridinylamino-octamethylene-9-aminoacridine. Similar experiments using 9-amino-6-azido-2-methoxyacridine indicate that this reagent recognizes changes in the DNA conformation induced by RNA polymerase binding, in relation to open complex formation.",
keywords = "Aminoacridines, Azides, Binding Sites, DNA, Bacterial/metabolism, DNA-Directed RNA Polymerases/metabolism, Escherichia coli/enzymology, Photochemistry, Promoter Regions, Genetic",
author = "O Buchardt and U Henriksen and Nielsen, {Peter E.}",
year = "1988",
month = "7",
day = "11",
language = "English",
volume = "16",
pages = "5755--70",
journal = "Nucleic Acids Research",
issn = "0305-1048",
publisher = "Oxford University Press",
number = "13",

}

RIS

TY - JOUR

T1 - Photocleavage of DNA and photofootprinting of E. coli RNA polymerase bound to promoter DNA by azido-9-acridinylamines

AU - Buchardt, O

AU - Henriksen, U

AU - Nielsen, Peter E.

PY - 1988/7/11

Y1 - 1988/7/11

N2 - The long-wavelength ultraviolet (lambda approximately 420 nm) radiation induced reaction between 6-azido-2-methoxy-9-acridinylamines and supercoiled plasmid DNA results in single strand scissions and formation of covalent adducts (ratio approximately 1:10). By treating azidoacridine-photomodified DNA with piperidine at 90 degrees C, additional strand scissions are observed in a complex sequence dependent manner with an overall preference for T greater than or equal to G greater than C much greater than A. The resulting DNA fragments migrate as 5'-phosphates in polyacrylamide gels. Photofootprinting of the binding site of RNA-polymerase on promoter DNA is demonstrated with an azido-9-acridinylamino-octamethylene-9-aminoacridine. Similar experiments using 9-amino-6-azido-2-methoxyacridine indicate that this reagent recognizes changes in the DNA conformation induced by RNA polymerase binding, in relation to open complex formation.

AB - The long-wavelength ultraviolet (lambda approximately 420 nm) radiation induced reaction between 6-azido-2-methoxy-9-acridinylamines and supercoiled plasmid DNA results in single strand scissions and formation of covalent adducts (ratio approximately 1:10). By treating azidoacridine-photomodified DNA with piperidine at 90 degrees C, additional strand scissions are observed in a complex sequence dependent manner with an overall preference for T greater than or equal to G greater than C much greater than A. The resulting DNA fragments migrate as 5'-phosphates in polyacrylamide gels. Photofootprinting of the binding site of RNA-polymerase on promoter DNA is demonstrated with an azido-9-acridinylamino-octamethylene-9-aminoacridine. Similar experiments using 9-amino-6-azido-2-methoxyacridine indicate that this reagent recognizes changes in the DNA conformation induced by RNA polymerase binding, in relation to open complex formation.

KW - Aminoacridines

KW - Azides

KW - Binding Sites

KW - DNA, Bacterial/metabolism

KW - DNA-Directed RNA Polymerases/metabolism

KW - Escherichia coli/enzymology

KW - Photochemistry

KW - Promoter Regions, Genetic

M3 - Journal article

C2 - 3041368

VL - 16

SP - 5755

EP - 5770

JO - Nucleic Acids Research

JF - Nucleic Acids Research

SN - 0305-1048

IS - 13

ER -

ID: 203631338