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Photosynthetic and transcriptional responses of the marine diatom Thalassiosira pseudonana to the combined effect of temperature stress and copper exposure

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  • Priscilla T. Y. Leung
  • Andy Xianliang Yi
  • Jack C. H. Ip
  • Mak, Sarah
  • Kenneth M. Y. Leung

A 96-h exposure experiment was conducted to elucidate the toxicity responses of the marine diatom Thalassiosira pseudonana upon exposure to different temperatures and copper (Cu) concentrations. Three Cu treatments (seawater control; 200μg/L Cu, EC50 for the yield at 25°C; and 1000μg/L Cu, EC50 for growth inhibition at 25°C) were conducted against four temperatures (10°C, 15°C, 25°C and 30°C). Growth rate and photosynthetic responses showed a significant interacting thermal-chemical effect with strong synergistic responses observed at 30°C treatments. Expression of heat shock protein (hsp) was positively modulated by increasing temperatures. Hsp 90, hsp90-2 and sit1 (related to silica shell formation) were highly expressed at 30°C under 1000μg/L Cu, while the genes encoding light harvesting proteins (3HfcpA and 3HfcpB) and silaffin precursor sil3 were significantly up-regulated at 15°C under 200μg/L Cu. Our results indicated an increase Cu toxicity to T. pseudonana under high temperature and Cu dose.

OriginalsprogEngelsk
TidsskriftMarine Pollution Bulletin
Vol/bind124
Udgave nummer2
Sider (fra-til)938-945
Antal sider8
ISSN0025-326X
DOI
StatusUdgivet - 30 nov. 2017

ID: 178742174