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pH-rate profiles of L-arabinitol 4-dehydrogenase from Hypocrea jecorina and its application in L-xylulose production

Publikation: Bidrag til tidsskriftLetterForskningfagfællebedømt

Standard

pH-rate profiles of L-arabinitol 4-dehydrogenase from Hypocrea jecorina and its application in L-xylulose production. / Tiwari, Manish Kumar; Singh, Raushan Kumar; Gao, Hui; Kim, Taesu; Chang, Suhwan; Kim, Han S; Lee, Jung-Kul.

I: Bioorganic & Medicinal Chemistry Letters, Bind 24, Nr. 1, 2014, s. 173-176.

Publikation: Bidrag til tidsskriftLetterForskningfagfællebedømt

Harvard

Tiwari, MK, Singh, RK, Gao, H, Kim, T, Chang, S, Kim, HS & Lee, J-K 2014, 'pH-rate profiles of L-arabinitol 4-dehydrogenase from Hypocrea jecorina and its application in L-xylulose production', Bioorganic & Medicinal Chemistry Letters, bind 24, nr. 1, s. 173-176. https://doi.org/10.1016/j.bmcl.2013.11.047

APA

Tiwari, M. K., Singh, R. K., Gao, H., Kim, T., Chang, S., Kim, H. S., & Lee, J-K. (2014). pH-rate profiles of L-arabinitol 4-dehydrogenase from Hypocrea jecorina and its application in L-xylulose production. Bioorganic & Medicinal Chemistry Letters, 24(1), 173-176. https://doi.org/10.1016/j.bmcl.2013.11.047

Vancouver

Tiwari MK, Singh RK, Gao H, Kim T, Chang S, Kim HS o.a. pH-rate profiles of L-arabinitol 4-dehydrogenase from Hypocrea jecorina and its application in L-xylulose production. Bioorganic & Medicinal Chemistry Letters. 2014;24(1):173-176. https://doi.org/10.1016/j.bmcl.2013.11.047

Author

Tiwari, Manish Kumar ; Singh, Raushan Kumar ; Gao, Hui ; Kim, Taesu ; Chang, Suhwan ; Kim, Han S ; Lee, Jung-Kul. / pH-rate profiles of L-arabinitol 4-dehydrogenase from Hypocrea jecorina and its application in L-xylulose production. I: Bioorganic & Medicinal Chemistry Letters. 2014 ; Bind 24, Nr. 1. s. 173-176.

Bibtex

@article{d6b678ddeca440a0acae1026cd9f2b28,
title = "pH-rate profiles of L-arabinitol 4-dehydrogenase from Hypocrea jecorina and its application in L-xylulose production",
abstract = "l-Arabinitol 4-dehydrogenase (LAD) from Hypocrea jecorina (HjLAD) was cloned and overexpressed in Escherichia coli BL21 (DE3). The kinetics of l-arabinitol oxidation by NAD(+), catalyzed by HjLAD, was studied within the pH range of 7.0-9.5 at 25°C. The turnover number (kcat) and the catalytic efficiency (kcat/Km) were 4200min(-1) and 290mM(-1)min(-1), respectively. HjLAD showed the highest turnover number and catalytic efficiency among all previously characterized LADs. In further application of HjLAD, rare l-sugar l-xylulose was produced by the enzymatic oxidation of arabinitol to give a yield of approximately 86{\%}.",
keywords = "Biocatalysis, Hydrogen-Ion Concentration, Hypocrea, Kinetics, Models, Molecular, Recombinant Proteins, Sugar Alcohol Dehydrogenases, Xylulose",
author = "Tiwari, {Manish Kumar} and Singh, {Raushan Kumar} and Hui Gao and Taesu Kim and Suhwan Chang and Kim, {Han S} and Jung-Kul Lee",
note = "Copyright {\circledC} 2013 Elsevier Ltd. All rights reserved.",
year = "2014",
doi = "10.1016/j.bmcl.2013.11.047",
language = "English",
volume = "24",
pages = "173--176",
journal = "Bioorganic & Medicinal Chemistry Letters",
issn = "0960-894X",
publisher = "Pergamon Press",
number = "1",

}

RIS

TY - JOUR

T1 - pH-rate profiles of L-arabinitol 4-dehydrogenase from Hypocrea jecorina and its application in L-xylulose production

AU - Tiwari, Manish Kumar

AU - Singh, Raushan Kumar

AU - Gao, Hui

AU - Kim, Taesu

AU - Chang, Suhwan

AU - Kim, Han S

AU - Lee, Jung-Kul

N1 - Copyright © 2013 Elsevier Ltd. All rights reserved.

PY - 2014

Y1 - 2014

N2 - l-Arabinitol 4-dehydrogenase (LAD) from Hypocrea jecorina (HjLAD) was cloned and overexpressed in Escherichia coli BL21 (DE3). The kinetics of l-arabinitol oxidation by NAD(+), catalyzed by HjLAD, was studied within the pH range of 7.0-9.5 at 25°C. The turnover number (kcat) and the catalytic efficiency (kcat/Km) were 4200min(-1) and 290mM(-1)min(-1), respectively. HjLAD showed the highest turnover number and catalytic efficiency among all previously characterized LADs. In further application of HjLAD, rare l-sugar l-xylulose was produced by the enzymatic oxidation of arabinitol to give a yield of approximately 86%.

AB - l-Arabinitol 4-dehydrogenase (LAD) from Hypocrea jecorina (HjLAD) was cloned and overexpressed in Escherichia coli BL21 (DE3). The kinetics of l-arabinitol oxidation by NAD(+), catalyzed by HjLAD, was studied within the pH range of 7.0-9.5 at 25°C. The turnover number (kcat) and the catalytic efficiency (kcat/Km) were 4200min(-1) and 290mM(-1)min(-1), respectively. HjLAD showed the highest turnover number and catalytic efficiency among all previously characterized LADs. In further application of HjLAD, rare l-sugar l-xylulose was produced by the enzymatic oxidation of arabinitol to give a yield of approximately 86%.

KW - Biocatalysis

KW - Hydrogen-Ion Concentration

KW - Hypocrea

KW - Kinetics

KW - Models, Molecular

KW - Recombinant Proteins

KW - Sugar Alcohol Dehydrogenases

KW - Xylulose

U2 - 10.1016/j.bmcl.2013.11.047

DO - 10.1016/j.bmcl.2013.11.047

M3 - Letter

C2 - 24342239

VL - 24

SP - 173

EP - 176

JO - Bioorganic & Medicinal Chemistry Letters

JF - Bioorganic & Medicinal Chemistry Letters

SN - 0960-894X

IS - 1

ER -

ID: 162607715