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Porcine lung surfactant protein B gene (SFTPB): cDNA sequencing, chromosomal location, and expression studies

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Porcine lung surfactant protein B gene (SFTPB) : cDNA sequencing, chromosomal location, and expression studies. / Cirera Salicio, Susanna; Fredholm, Merete.

I: Animal Biotechnology (Print Edition), Bind 19, Nr. 3, 2008, s. 127-137.

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningfagfællebedømt

Harvard

Cirera Salicio, S & Fredholm, M 2008, 'Porcine lung surfactant protein B gene (SFTPB): cDNA sequencing, chromosomal location, and expression studies', Animal Biotechnology (Print Edition), bind 19, nr. 3, s. 127-137. https://doi.org/10.1080/10495390801977584

APA

Cirera Salicio, S., & Fredholm, M. (2008). Porcine lung surfactant protein B gene (SFTPB): cDNA sequencing, chromosomal location, and expression studies. Animal Biotechnology (Print Edition), 19(3), 127-137. https://doi.org/10.1080/10495390801977584

Vancouver

Cirera Salicio S, Fredholm M. Porcine lung surfactant protein B gene (SFTPB): cDNA sequencing, chromosomal location, and expression studies. Animal Biotechnology (Print Edition). 2008;19(3):127-137. https://doi.org/10.1080/10495390801977584

Author

Cirera Salicio, Susanna ; Fredholm, Merete. / Porcine lung surfactant protein B gene (SFTPB) : cDNA sequencing, chromosomal location, and expression studies. I: Animal Biotechnology (Print Edition). 2008 ; Bind 19, Nr. 3. s. 127-137.

Bibtex

@article{491be610a1c411ddb6ae000ea68e967b,
title = "Porcine lung surfactant protein B gene (SFTPB): cDNA sequencing, chromosomal location, and expression studies",
abstract = "The porcine surfactant protein B (SFTPB) is a single copy gene on chromosome 3. Three different cDNAs for the SFTPB have been isolated and sequenced. Nucleotide sequence comparison revealed six nonsynonymous single nucleotide polymorphisms (SNPs), four synonymous SNPs and an in-frame deletion of 69 bp in the region coding for the active protein. Northern analysis showed lung-specific expression of three different isoforms of the SFTPB transcript. The expression level for the SFTPB gene is low in 50 days-old fetus and it increases during lung development. Quantitative real-time polymerase chain reaction (qPCR) showed significant down regulation of the SFTPB in pigs with Actinobacillus pleuropneumoniae lung infection.",
keywords = "Former LIFE faculty, Actinobacillus pleuropneumoniae, qPCR, SFTPB, SNP, Sus crofa",
author = "{Cirera Salicio}, Susanna and Merete Fredholm",
year = "2008",
doi = "10.1080/10495390801977584",
language = "English",
volume = "19",
pages = "127--137",
journal = "Animal Biotechnology (Print Edition)",
issn = "1049-5398",
publisher = "Taylor & Francis",
number = "3",

}

RIS

TY - JOUR

T1 - Porcine lung surfactant protein B gene (SFTPB)

T2 - cDNA sequencing, chromosomal location, and expression studies

AU - Cirera Salicio, Susanna

AU - Fredholm, Merete

PY - 2008

Y1 - 2008

N2 - The porcine surfactant protein B (SFTPB) is a single copy gene on chromosome 3. Three different cDNAs for the SFTPB have been isolated and sequenced. Nucleotide sequence comparison revealed six nonsynonymous single nucleotide polymorphisms (SNPs), four synonymous SNPs and an in-frame deletion of 69 bp in the region coding for the active protein. Northern analysis showed lung-specific expression of three different isoforms of the SFTPB transcript. The expression level for the SFTPB gene is low in 50 days-old fetus and it increases during lung development. Quantitative real-time polymerase chain reaction (qPCR) showed significant down regulation of the SFTPB in pigs with Actinobacillus pleuropneumoniae lung infection.

AB - The porcine surfactant protein B (SFTPB) is a single copy gene on chromosome 3. Three different cDNAs for the SFTPB have been isolated and sequenced. Nucleotide sequence comparison revealed six nonsynonymous single nucleotide polymorphisms (SNPs), four synonymous SNPs and an in-frame deletion of 69 bp in the region coding for the active protein. Northern analysis showed lung-specific expression of three different isoforms of the SFTPB transcript. The expression level for the SFTPB gene is low in 50 days-old fetus and it increases during lung development. Quantitative real-time polymerase chain reaction (qPCR) showed significant down regulation of the SFTPB in pigs with Actinobacillus pleuropneumoniae lung infection.

KW - Former LIFE faculty

KW - Actinobacillus pleuropneumoniae

KW - qPCR

KW - SFTPB

KW - SNP

KW - Sus crofa

U2 - 10.1080/10495390801977584

DO - 10.1080/10495390801977584

M3 - Journal article

C2 - 18607785

VL - 19

SP - 127

EP - 137

JO - Animal Biotechnology (Print Edition)

JF - Animal Biotechnology (Print Edition)

SN - 1049-5398

IS - 3

ER -

ID: 8113536