Forskning ved Københavns Universitet - Københavns Universitet

Forside

Positive association between cholesterol in human seminal plasma and sperm counts: results from a cross-sectional cohort study and immunohistochemical investigations

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningfagfællebedømt

Standard

Positive association between cholesterol in human seminal plasma and sperm counts : results from a cross-sectional cohort study and immunohistochemical investigations. / de Neergaard, R.; Nielsen, J. E.; Jørgensen, A.; Toft, B. G.; Goetze, J. P.; Jørgensen, N.

I: Andrology, Bind 6, Nr. 6, 2018, s. 817-828.

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningfagfællebedømt

Harvard

de Neergaard, R, Nielsen, JE, Jørgensen, A, Toft, BG, Goetze, JP & Jørgensen, N 2018, 'Positive association between cholesterol in human seminal plasma and sperm counts: results from a cross-sectional cohort study and immunohistochemical investigations', Andrology, bind 6, nr. 6, s. 817-828. https://doi.org/10.1111/andr.12532

APA

de Neergaard, R., Nielsen, J. E., Jørgensen, A., Toft, B. G., Goetze, J. P., & Jørgensen, N. (2018). Positive association between cholesterol in human seminal plasma and sperm counts: results from a cross-sectional cohort study and immunohistochemical investigations. Andrology, 6(6), 817-828. https://doi.org/10.1111/andr.12532

Vancouver

de Neergaard R, Nielsen JE, Jørgensen A, Toft BG, Goetze JP, Jørgensen N. Positive association between cholesterol in human seminal plasma and sperm counts: results from a cross-sectional cohort study and immunohistochemical investigations. Andrology. 2018;6(6):817-828. https://doi.org/10.1111/andr.12532

Author

de Neergaard, R. ; Nielsen, J. E. ; Jørgensen, A. ; Toft, B. G. ; Goetze, J. P. ; Jørgensen, N. / Positive association between cholesterol in human seminal plasma and sperm counts : results from a cross-sectional cohort study and immunohistochemical investigations. I: Andrology. 2018 ; Bind 6, Nr. 6. s. 817-828.

Bibtex

@article{9ff68c9b5fe343569993e905184d981e,
title = "Positive association between cholesterol in human seminal plasma and sperm counts: results from a cross-sectional cohort study and immunohistochemical investigations",
abstract = "Background: Cholesterol is essential for cell membrane stability, permeability, and fluidity. Cholesterol is present in seminal plasma, but whether a relationship between the level of cholesterol in seminal plasma and semen quality exists remains to be elucidated. Objectives: To explore the association between cholesterol levels in seminal plasma and serum cholesterols, semen quality, and serum reproductive hormones. Secondly, to explore whether the associations are biologically plausible. Materials and Methods: An association study between cholesterol levels in seminal plasma and semen quality in 403 men, median age 19 years, from the general population. Additionally, an immunohistochemical evaluation of proteins involved in cholesterol metabolism and transport in tissues from the male reproductive tract (testis, epididymis, prostate, and seminal vesicle). Tissue specimens were investigated by immunohistochemistry for markers of cholesterol metabolism and transport (ABCA1, ABCG1, CYP11A1, CYP51A1, HMGCR, LAL, LCAT, LDLR, and SOAT1). Results: Trend analyses showed that total amount of total cholesterol in seminal plasma was positively associated with sperm concentration, total sperm count, sperm motility, and morphology (all p < 0.008, adjusted). Cholesterol concentrations in seminal plasma were neither associated with serum cholesterol and lipid levels nor serum reproductive hormone (FSH, LH, testosterone, estradiol, sex-hormone-binding globulin, inhibin b) levels. All investigated markers of cholesterol metabolism and transport were expressed in the investigated tissue specimens to varying degrees. Discussion: Seminal plasma level of cholesterol was positively associated with semen parameters. The presence of proteins and enzymes involved in cholesterol metabolism in Leydig cells, Sertoli cells, and maturing germ cells in the seminiferous tubules supports the view that cholesterol may be important for spermatogenesis. Conclusion: Cholesterol level in seminal plasma may be an indicator of semen quality. Investigations are needed to corroborate or refute our findings and to clarify the exact role of cholesterols for semen quality.",
keywords = "cholesterol, male reproduction, semen quality, spermatozoa, testis",
author = "{de Neergaard}, R. and Nielsen, {J. E.} and A. J{\o}rgensen and Toft, {B. G.} and Goetze, {J. P.} and N. J{\o}rgensen",
year = "2018",
doi = "10.1111/andr.12532",
language = "English",
volume = "6",
pages = "817--828",
journal = "Andrology",
issn = "2047-2919",
publisher = "Wiley-Blackwell",
number = "6",

}

RIS

TY - JOUR

T1 - Positive association between cholesterol in human seminal plasma and sperm counts

T2 - results from a cross-sectional cohort study and immunohistochemical investigations

AU - de Neergaard, R.

AU - Nielsen, J. E.

AU - Jørgensen, A.

AU - Toft, B. G.

AU - Goetze, J. P.

AU - Jørgensen, N.

PY - 2018

Y1 - 2018

N2 - Background: Cholesterol is essential for cell membrane stability, permeability, and fluidity. Cholesterol is present in seminal plasma, but whether a relationship between the level of cholesterol in seminal plasma and semen quality exists remains to be elucidated. Objectives: To explore the association between cholesterol levels in seminal plasma and serum cholesterols, semen quality, and serum reproductive hormones. Secondly, to explore whether the associations are biologically plausible. Materials and Methods: An association study between cholesterol levels in seminal plasma and semen quality in 403 men, median age 19 years, from the general population. Additionally, an immunohistochemical evaluation of proteins involved in cholesterol metabolism and transport in tissues from the male reproductive tract (testis, epididymis, prostate, and seminal vesicle). Tissue specimens were investigated by immunohistochemistry for markers of cholesterol metabolism and transport (ABCA1, ABCG1, CYP11A1, CYP51A1, HMGCR, LAL, LCAT, LDLR, and SOAT1). Results: Trend analyses showed that total amount of total cholesterol in seminal plasma was positively associated with sperm concentration, total sperm count, sperm motility, and morphology (all p < 0.008, adjusted). Cholesterol concentrations in seminal plasma were neither associated with serum cholesterol and lipid levels nor serum reproductive hormone (FSH, LH, testosterone, estradiol, sex-hormone-binding globulin, inhibin b) levels. All investigated markers of cholesterol metabolism and transport were expressed in the investigated tissue specimens to varying degrees. Discussion: Seminal plasma level of cholesterol was positively associated with semen parameters. The presence of proteins and enzymes involved in cholesterol metabolism in Leydig cells, Sertoli cells, and maturing germ cells in the seminiferous tubules supports the view that cholesterol may be important for spermatogenesis. Conclusion: Cholesterol level in seminal plasma may be an indicator of semen quality. Investigations are needed to corroborate or refute our findings and to clarify the exact role of cholesterols for semen quality.

AB - Background: Cholesterol is essential for cell membrane stability, permeability, and fluidity. Cholesterol is present in seminal plasma, but whether a relationship between the level of cholesterol in seminal plasma and semen quality exists remains to be elucidated. Objectives: To explore the association between cholesterol levels in seminal plasma and serum cholesterols, semen quality, and serum reproductive hormones. Secondly, to explore whether the associations are biologically plausible. Materials and Methods: An association study between cholesterol levels in seminal plasma and semen quality in 403 men, median age 19 years, from the general population. Additionally, an immunohistochemical evaluation of proteins involved in cholesterol metabolism and transport in tissues from the male reproductive tract (testis, epididymis, prostate, and seminal vesicle). Tissue specimens were investigated by immunohistochemistry for markers of cholesterol metabolism and transport (ABCA1, ABCG1, CYP11A1, CYP51A1, HMGCR, LAL, LCAT, LDLR, and SOAT1). Results: Trend analyses showed that total amount of total cholesterol in seminal plasma was positively associated with sperm concentration, total sperm count, sperm motility, and morphology (all p < 0.008, adjusted). Cholesterol concentrations in seminal plasma were neither associated with serum cholesterol and lipid levels nor serum reproductive hormone (FSH, LH, testosterone, estradiol, sex-hormone-binding globulin, inhibin b) levels. All investigated markers of cholesterol metabolism and transport were expressed in the investigated tissue specimens to varying degrees. Discussion: Seminal plasma level of cholesterol was positively associated with semen parameters. The presence of proteins and enzymes involved in cholesterol metabolism in Leydig cells, Sertoli cells, and maturing germ cells in the seminiferous tubules supports the view that cholesterol may be important for spermatogenesis. Conclusion: Cholesterol level in seminal plasma may be an indicator of semen quality. Investigations are needed to corroborate or refute our findings and to clarify the exact role of cholesterols for semen quality.

KW - cholesterol

KW - male reproduction

KW - semen quality

KW - spermatozoa

KW - testis

U2 - 10.1111/andr.12532

DO - 10.1111/andr.12532

M3 - Journal article

C2 - 30182437

AN - SCOPUS:85052934667

VL - 6

SP - 817

EP - 828

JO - Andrology

JF - Andrology

SN - 2047-2919

IS - 6

ER -

ID: 221691069