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Proliferation and apoptosis of lamina propria CD4+ T cells from scid mice with inflammatory bowel disease

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  • S Bregenholt
  • J Reimann
  • Mogens Helweg Claesson
Scid mice transplanted with low numbers of syngeneic CD4+ T cells, develop a chronic and lethal inflammatory bowel disease (IBD) within 4-6 months. We have used in vivo 5-bromo2-deoxy-uridine (BrdU) labeling to assess the proliferation of lamina propria-derived CD4+ T cells in diseased scid mice. The hourly rate of renewal of colonic lamina propria CD4+ T cells in diseased mice was 7% compared with 1.5% in normal BALB/c control mice. Transplantation of scid mice with in vitro activated CD4+ T cells accelerated the disease onset and development in a cell dose-dependent fashion when compared with non-activated CD4+ T cells. In pulse-chase experiments it was shown that BrdU-labeled cells disappeared rapidly from the lamina propria of diseased mice. DNA analysis revealed that this was due to the presence of nearly four times as many apoptotic CD4+ T cells in diseased than in control mice. Further analyses showed that the apoptotic lamina propria CD4+ T cells were derived from cells having entered the cell cycle within the previous 8 h. These data clearly demonstrate that vigorous CD4+ T cell proliferation and death are involved throughout the course of IBD.
OriginalsprogEngelsk
TidsskriftEuropean Journal of Immunology
Vol/bind28
Udgave nummer11
Sider (fra-til)3655-63
Antal sider9
ISSN0014-2980
DOI
StatusUdgivet - 1 nov. 1998

ID: 32637467