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Proliferation and apoptosis of lamina propria CD4+ T cells from scid mice with inflammatory bowel disease

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Proliferation and apoptosis of lamina propria CD4+ T cells from scid mice with inflammatory bowel disease. / Bregenholt, S; Reimann, J; Claesson, Mogens Helweg.

I: European Journal of Immunology, Bind 28, Nr. 11, 01.11.1998, s. 3655-63.

Publikation: Bidrag til tidsskriftTidsskriftartikel

Harvard

Bregenholt, S, Reimann, J & Claesson, MH 1998, 'Proliferation and apoptosis of lamina propria CD4+ T cells from scid mice with inflammatory bowel disease', European Journal of Immunology, bind 28, nr. 11, s. 3655-63. https://doi.org/10.1002/(SICI)1521-4141(199811)28:11<3655::AID-IMMU3655>3.0.CO;2-9

APA

Bregenholt, S., Reimann, J., & Claesson, M. H. (1998). Proliferation and apoptosis of lamina propria CD4+ T cells from scid mice with inflammatory bowel disease. European Journal of Immunology, 28(11), 3655-63. https://doi.org/10.1002/(SICI)1521-4141(199811)28:11<3655::AID-IMMU3655>3.0.CO;2-9

Vancouver

Bregenholt S, Reimann J, Claesson MH. Proliferation and apoptosis of lamina propria CD4+ T cells from scid mice with inflammatory bowel disease. European Journal of Immunology. 1998 nov 1;28(11):3655-63. https://doi.org/10.1002/(SICI)1521-4141(199811)28:11<3655::AID-IMMU3655>3.0.CO;2-9

Author

Bregenholt, S ; Reimann, J ; Claesson, Mogens Helweg. / Proliferation and apoptosis of lamina propria CD4+ T cells from scid mice with inflammatory bowel disease. I: European Journal of Immunology. 1998 ; Bind 28, Nr. 11. s. 3655-63.

Bibtex

@article{3fd62dda9b3a4eb5b7f23d12ed04e0da,
title = "Proliferation and apoptosis of lamina propria CD4+ T cells from scid mice with inflammatory bowel disease",
abstract = "Scid mice transplanted with low numbers of syngeneic CD4+ T cells, develop a chronic and lethal inflammatory bowel disease (IBD) within 4-6 months. We have used in vivo 5-bromo2-deoxy-uridine (BrdU) labeling to assess the proliferation of lamina propria-derived CD4+ T cells in diseased scid mice. The hourly rate of renewal of colonic lamina propria CD4+ T cells in diseased mice was 7{\%} compared with 1.5{\%} in normal BALB/c control mice. Transplantation of scid mice with in vitro activated CD4+ T cells accelerated the disease onset and development in a cell dose-dependent fashion when compared with non-activated CD4+ T cells. In pulse-chase experiments it was shown that BrdU-labeled cells disappeared rapidly from the lamina propria of diseased mice. DNA analysis revealed that this was due to the presence of nearly four times as many apoptotic CD4+ T cells in diseased than in control mice. Further analyses showed that the apoptotic lamina propria CD4+ T cells were derived from cells having entered the cell cycle within the previous 8 h. These data clearly demonstrate that vigorous CD4+ T cell proliferation and death are involved throughout the course of IBD.",
keywords = "Animals, Apoptosis, Bromodeoxyuridine, CD4-Positive T-Lymphocytes, Inflammatory Bowel Diseases, Intestinal Mucosa, Lymphocyte Activation, Mice, Mice, Inbred BALB C, Mice, SCID, Rats",
author = "S Bregenholt and J Reimann and Claesson, {Mogens Helweg}",
year = "1998",
month = "11",
day = "1",
doi = "10.1002/(SICI)1521-4141(199811)28:11<3655::AID-IMMU3655>3.0.CO;2-9",
language = "English",
volume = "28",
pages = "3655--63",
journal = "European Journal of Immunology",
issn = "0014-2980",
publisher = "Wiley - V C H Verlag GmbH & Co. KGaA",
number = "11",

}

RIS

TY - JOUR

T1 - Proliferation and apoptosis of lamina propria CD4+ T cells from scid mice with inflammatory bowel disease

AU - Bregenholt, S

AU - Reimann, J

AU - Claesson, Mogens Helweg

PY - 1998/11/1

Y1 - 1998/11/1

N2 - Scid mice transplanted with low numbers of syngeneic CD4+ T cells, develop a chronic and lethal inflammatory bowel disease (IBD) within 4-6 months. We have used in vivo 5-bromo2-deoxy-uridine (BrdU) labeling to assess the proliferation of lamina propria-derived CD4+ T cells in diseased scid mice. The hourly rate of renewal of colonic lamina propria CD4+ T cells in diseased mice was 7% compared with 1.5% in normal BALB/c control mice. Transplantation of scid mice with in vitro activated CD4+ T cells accelerated the disease onset and development in a cell dose-dependent fashion when compared with non-activated CD4+ T cells. In pulse-chase experiments it was shown that BrdU-labeled cells disappeared rapidly from the lamina propria of diseased mice. DNA analysis revealed that this was due to the presence of nearly four times as many apoptotic CD4+ T cells in diseased than in control mice. Further analyses showed that the apoptotic lamina propria CD4+ T cells were derived from cells having entered the cell cycle within the previous 8 h. These data clearly demonstrate that vigorous CD4+ T cell proliferation and death are involved throughout the course of IBD.

AB - Scid mice transplanted with low numbers of syngeneic CD4+ T cells, develop a chronic and lethal inflammatory bowel disease (IBD) within 4-6 months. We have used in vivo 5-bromo2-deoxy-uridine (BrdU) labeling to assess the proliferation of lamina propria-derived CD4+ T cells in diseased scid mice. The hourly rate of renewal of colonic lamina propria CD4+ T cells in diseased mice was 7% compared with 1.5% in normal BALB/c control mice. Transplantation of scid mice with in vitro activated CD4+ T cells accelerated the disease onset and development in a cell dose-dependent fashion when compared with non-activated CD4+ T cells. In pulse-chase experiments it was shown that BrdU-labeled cells disappeared rapidly from the lamina propria of diseased mice. DNA analysis revealed that this was due to the presence of nearly four times as many apoptotic CD4+ T cells in diseased than in control mice. Further analyses showed that the apoptotic lamina propria CD4+ T cells were derived from cells having entered the cell cycle within the previous 8 h. These data clearly demonstrate that vigorous CD4+ T cell proliferation and death are involved throughout the course of IBD.

KW - Animals

KW - Apoptosis

KW - Bromodeoxyuridine

KW - CD4-Positive T-Lymphocytes

KW - Inflammatory Bowel Diseases

KW - Intestinal Mucosa

KW - Lymphocyte Activation

KW - Mice

KW - Mice, Inbred BALB C

KW - Mice, SCID

KW - Rats

U2 - 10.1002/(SICI)1521-4141(199811)28:11<3655::AID-IMMU3655>3.0.CO;2-9

DO - 10.1002/(SICI)1521-4141(199811)28:11<3655::AID-IMMU3655>3.0.CO;2-9

M3 - Journal article

C2 - 9842908

VL - 28

SP - 3655

EP - 3663

JO - European Journal of Immunology

JF - European Journal of Immunology

SN - 0014-2980

IS - 11

ER -

ID: 32637467