Forskning ved Københavns Universitet - Københavns Universitet

Forside

Protein aggregation capture on microparticles enables multi-purpose proteomics sample preparation

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningfagfællebedømt

Universal proteomics sample preparation is challenging due to the high heterogeneity of biological samples. Here we describe a novel mechanism that exploits the inherent instability of denatured proteins for non-specific immobilization on microparticles by protein aggregation capture. To demonstrate the general applicability of this mechanism, we analyzed phosphoproteomes, tissue proteomes, and interaction proteomes as well as dilute secretomes. The findings presents a practical, sensitive and cost-effective proteomics sample preparation method.

OriginalsprogEngelsk
TidsskriftMolecular and Cellular Proteomics
Vol/bind18
Udgave nummer5
Sider (fra-til)1027-1035
ISSN1535-9476
DOI
StatusUdgivet - 2019

Antal downloads er baseret på statistik fra Google Scholar og www.ku.dk


Ingen data tilgængelig

ID: 214465266