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Recurrent DNA inversion rearrangements in the human genome

Publikation: Bidrag til tidsskriftTidsskriftartikelForskning

  • Margarita Flores
  • Lucía Morales
  • Claudia Gonzaga-Jauregui
  • Rocío Domínguez-Vidaña
  • Cinthya Zepeda
  • Omar Yañez
  • María Gutiérrez
  • Tzitziki Lemus
  • David Valle
  • Maria del Carmen Avila Arcos
  • Daniel Blanco
  • Sofía Medina-Ruiz
  • Karla Meza
  • Erandi Ayala
  • Delfino García
  • Patricia Bustos
  • Víctor González
  • Lourdes Girard
  • Teresa Tusie-Luna
  • Guillermo Dávila
  • Og 2 flere
  • Rafael Palacios
  • Maria del Carmen Avila Arcos
Several lines of evidence suggest that reiterated sequences in the human genome are targets for nonallelic homologous recombination (NAHR), which facilitates genomic rearrangements. We have used a PCR-based approach to identify breakpoint regions of rearranged structures in the human genome. In particular, we have identified intrachromosomal identical repeats that are located in reverse orientation, which may lead to chromosomal inversions. A bioinformatic workflow pathway to select appropriate regions for analysis was developed. Three such regions overlapping with known human genes, located on chromosomes 3, 15, and 19, were analyzed. The relative proportion of wild-type to rearranged structures was determined in DNA samples from blood obtained from different, unrelated individuals. The results obtained indicate that recurrent genomic rearrangements occur at relatively high frequency in somatic cells. Interestingly, the rearrangements studied were significantly more abundant in adults than in newborn individuals, suggesting that such DNA rearrangements might start to appear during embryogenesis or fetal life and continue to accumulate after birth. The relevance of our results in regard to human genomic variation is discussed.
OriginalsprogEngelsk
TidsskriftProceedings of the National Academy of Sciences of the United States of America
Vol/bind104
Udgave nummer15
Sider (fra-til)6099-106
Antal sider8
ISSN0027-8424
DOI
StatusUdgivet - 10 apr. 2007

ID: 46089961