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Recurrent requirement for the m6A-ECT2/ECT3/ECT4 axis in the control of cell proliferation during plant organogenesis

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Standard

Recurrent requirement for the m6A-ECT2/ECT3/ECT4 axis in the control of cell proliferation during plant organogenesis. / Arribas-Hernández, Laura; Simonini, Sara; Hansen, Mathias Henning; Botterweg Paredes, Esther; Bressendorff, Simon; Dong, Yang; Østergaard, Lars; Brodersen, Peter.

I: Development, Bind 147, Nr. 14, dev189134, 2020.

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningfagfællebedømt

Harvard

Arribas-Hernández, L, Simonini, S, Hansen, MH, Botterweg Paredes, E, Bressendorff, S, Dong, Y, Østergaard, L & Brodersen, P 2020, 'Recurrent requirement for the m6A-ECT2/ECT3/ECT4 axis in the control of cell proliferation during plant organogenesis', Development, bind 147, nr. 14, dev189134. https://doi.org/10.1242/dev.189134

APA

Arribas-Hernández, L., Simonini, S., Hansen, M. H., Botterweg Paredes, E., Bressendorff, S., Dong, Y., ... Brodersen, P. (2020). Recurrent requirement for the m6A-ECT2/ECT3/ECT4 axis in the control of cell proliferation during plant organogenesis. Development, 147(14), [dev189134]. https://doi.org/10.1242/dev.189134

Vancouver

Arribas-Hernández L, Simonini S, Hansen MH, Botterweg Paredes E, Bressendorff S, Dong Y o.a. Recurrent requirement for the m6A-ECT2/ECT3/ECT4 axis in the control of cell proliferation during plant organogenesis. Development. 2020;147(14). dev189134. https://doi.org/10.1242/dev.189134

Author

Arribas-Hernández, Laura ; Simonini, Sara ; Hansen, Mathias Henning ; Botterweg Paredes, Esther ; Bressendorff, Simon ; Dong, Yang ; Østergaard, Lars ; Brodersen, Peter. / Recurrent requirement for the m6A-ECT2/ECT3/ECT4 axis in the control of cell proliferation during plant organogenesis. I: Development. 2020 ; Bind 147, Nr. 14.

Bibtex

@article{1068c9ce26694f76bbf683cb6e32c820,
title = "Recurrent requirement for the m6A-ECT2/ECT3/ECT4 axis in the control of cell proliferation during plant organogenesis",
abstract = "mRNA methylation at the N6-position of adenosine (m6A) enables multiple layers of post-transcriptional gene control, often via RNA-binding proteins that use a YT521-B homology (YTH) domain for specific m6A recognition. In Arabidopsis, normal leaf morphogenesis and rate of leaf formation require m6A and the YTH-domain proteins ECT2, ECT3 and ECT4. In this study, we show that ect2/ect3 and ect2/ect3/ect4 mutants also exhibit slow root and stem growth, slow flower formation, defective directionality of root growth, and aberrant flower and fruit morphology. In all cases, the m6A-binding site of ECT proteins is required for in vivo function. We also demonstrate that both m6A methyltransferase mutants and ect2/ect3/ect4 exhibit aberrant floral phyllotaxis. Consistent with the delayed organogenesis phenotypes, we observe particularly high expression of ECT2, ECT3 and ECT4 in rapidly dividing cells of organ primordia. Accordingly, ect2/ect3/ect4 mutants exhibit decreased rates of cell division in leaf and vascular primordia. Thus, the m6A-ECT2/ECT3/ECT4 axis is employed as a recurrent module to stimulate plant organogenesis, at least in part by enabling rapid cellular proliferation.",
keywords = "ECT2, ECT3, ECT4, m6A, Plant organogenesis, YTH domain",
author = "Laura Arribas-Hern{\'a}ndez and Sara Simonini and Hansen, {Mathias Henning} and {Botterweg Paredes}, Esther and Simon Bressendorff and Yang Dong and Lars {\O}stergaard and Peter Brodersen",
year = "2020",
doi = "10.1242/dev.189134",
language = "English",
volume = "147",
journal = "Development",
issn = "0950-1991",
publisher = "The Company of Biologists",
number = "14",

}

RIS

TY - JOUR

T1 - Recurrent requirement for the m6A-ECT2/ECT3/ECT4 axis in the control of cell proliferation during plant organogenesis

AU - Arribas-Hernández, Laura

AU - Simonini, Sara

AU - Hansen, Mathias Henning

AU - Botterweg Paredes, Esther

AU - Bressendorff, Simon

AU - Dong, Yang

AU - Østergaard, Lars

AU - Brodersen, Peter

PY - 2020

Y1 - 2020

N2 - mRNA methylation at the N6-position of adenosine (m6A) enables multiple layers of post-transcriptional gene control, often via RNA-binding proteins that use a YT521-B homology (YTH) domain for specific m6A recognition. In Arabidopsis, normal leaf morphogenesis and rate of leaf formation require m6A and the YTH-domain proteins ECT2, ECT3 and ECT4. In this study, we show that ect2/ect3 and ect2/ect3/ect4 mutants also exhibit slow root and stem growth, slow flower formation, defective directionality of root growth, and aberrant flower and fruit morphology. In all cases, the m6A-binding site of ECT proteins is required for in vivo function. We also demonstrate that both m6A methyltransferase mutants and ect2/ect3/ect4 exhibit aberrant floral phyllotaxis. Consistent with the delayed organogenesis phenotypes, we observe particularly high expression of ECT2, ECT3 and ECT4 in rapidly dividing cells of organ primordia. Accordingly, ect2/ect3/ect4 mutants exhibit decreased rates of cell division in leaf and vascular primordia. Thus, the m6A-ECT2/ECT3/ECT4 axis is employed as a recurrent module to stimulate plant organogenesis, at least in part by enabling rapid cellular proliferation.

AB - mRNA methylation at the N6-position of adenosine (m6A) enables multiple layers of post-transcriptional gene control, often via RNA-binding proteins that use a YT521-B homology (YTH) domain for specific m6A recognition. In Arabidopsis, normal leaf morphogenesis and rate of leaf formation require m6A and the YTH-domain proteins ECT2, ECT3 and ECT4. In this study, we show that ect2/ect3 and ect2/ect3/ect4 mutants also exhibit slow root and stem growth, slow flower formation, defective directionality of root growth, and aberrant flower and fruit morphology. In all cases, the m6A-binding site of ECT proteins is required for in vivo function. We also demonstrate that both m6A methyltransferase mutants and ect2/ect3/ect4 exhibit aberrant floral phyllotaxis. Consistent with the delayed organogenesis phenotypes, we observe particularly high expression of ECT2, ECT3 and ECT4 in rapidly dividing cells of organ primordia. Accordingly, ect2/ect3/ect4 mutants exhibit decreased rates of cell division in leaf and vascular primordia. Thus, the m6A-ECT2/ECT3/ECT4 axis is employed as a recurrent module to stimulate plant organogenesis, at least in part by enabling rapid cellular proliferation.

KW - ECT2

KW - ECT3

KW - ECT4

KW - m6A

KW - Plant organogenesis

KW - YTH domain

U2 - 10.1242/dev.189134

DO - 10.1242/dev.189134

M3 - Journal article

C2 - 32611605

AN - SCOPUS:85088680904

VL - 147

JO - Development

JF - Development

SN - 0950-1991

IS - 14

M1 - dev189134

ER -

ID: 247385735