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Reversed-phase high-performance liquid chromatographic analyses of insulin biosynthesis in isolated rat and mouse islets

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Standard

Reversed-phase high-performance liquid chromatographic analyses of insulin biosynthesis in isolated rat and mouse islets. / Linde, S; Hansen, Bruno A.; Welinder, B S; Nielsen, Jens Høiriis.

I: Journal of Chromatography A, Bind 462, 13.01.1989, s. 243-54.

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningfagfællebedømt

Harvard

Linde, S, Hansen, BA, Welinder, BS & Nielsen, JH 1989, 'Reversed-phase high-performance liquid chromatographic analyses of insulin biosynthesis in isolated rat and mouse islets', Journal of Chromatography A, bind 462, s. 243-54.

APA

Linde, S., Hansen, B. A., Welinder, B. S., & Nielsen, J. H. (1989). Reversed-phase high-performance liquid chromatographic analyses of insulin biosynthesis in isolated rat and mouse islets. Journal of Chromatography A, 462, 243-54.

Vancouver

Linde S, Hansen BA, Welinder BS, Nielsen JH. Reversed-phase high-performance liquid chromatographic analyses of insulin biosynthesis in isolated rat and mouse islets. Journal of Chromatography A. 1989 jan 13;462:243-54.

Author

Linde, S ; Hansen, Bruno A. ; Welinder, B S ; Nielsen, Jens Høiriis. / Reversed-phase high-performance liquid chromatographic analyses of insulin biosynthesis in isolated rat and mouse islets. I: Journal of Chromatography A. 1989 ; Bind 462. s. 243-54.

Bibtex

@article{82908786d91e46a39844053dc22c7406,
title = "Reversed-phase high-performance liquid chromatographic analyses of insulin biosynthesis in isolated rat and mouse islets",
abstract = "Two RP-HPLC systems were developed for the separation of the products of the conversion of proinsulin into insulin in rat and mouse islets, including proinsulin I and II. Peaks were identified by microsequencing and radiosequencing. It was confirmed that mouse C-peptide I has a two amino acid deletion compared to rat C-peptide I. A marked species difference in the ratio between insulin I and II was observed, i.e., 2:1 in the rat and 1:2 in the mouse. Pulse-chase experiments in rat islets have demonstrated that the ratio between insulin I and II in newly synthesized insulin is higher than that of the stored insulin, indicating a slower conversion rate of proinsulin II compared to proinsulin I.",
keywords = "Alleles, Amino Acid Sequence, Animals, C-Peptide, Chromatography, High Pressure Liquid, Insulin, Islets of Langerhans, Mice, Mice, Inbred Strains, Molecular Sequence Data, Proinsulin, Rats, Rats, Inbred Strains",
author = "S Linde and Hansen, {Bruno A.} and Welinder, {B S} and Nielsen, {Jens H{\o}iriis}",
year = "1989",
month = jan,
day = "13",
language = "English",
volume = "462",
pages = "243--54",
journal = "Journal of Chromatography A",
issn = "0021-9673",
publisher = "Elsevier",

}

RIS

TY - JOUR

T1 - Reversed-phase high-performance liquid chromatographic analyses of insulin biosynthesis in isolated rat and mouse islets

AU - Linde, S

AU - Hansen, Bruno A.

AU - Welinder, B S

AU - Nielsen, Jens Høiriis

PY - 1989/1/13

Y1 - 1989/1/13

N2 - Two RP-HPLC systems were developed for the separation of the products of the conversion of proinsulin into insulin in rat and mouse islets, including proinsulin I and II. Peaks were identified by microsequencing and radiosequencing. It was confirmed that mouse C-peptide I has a two amino acid deletion compared to rat C-peptide I. A marked species difference in the ratio between insulin I and II was observed, i.e., 2:1 in the rat and 1:2 in the mouse. Pulse-chase experiments in rat islets have demonstrated that the ratio between insulin I and II in newly synthesized insulin is higher than that of the stored insulin, indicating a slower conversion rate of proinsulin II compared to proinsulin I.

AB - Two RP-HPLC systems were developed for the separation of the products of the conversion of proinsulin into insulin in rat and mouse islets, including proinsulin I and II. Peaks were identified by microsequencing and radiosequencing. It was confirmed that mouse C-peptide I has a two amino acid deletion compared to rat C-peptide I. A marked species difference in the ratio between insulin I and II was observed, i.e., 2:1 in the rat and 1:2 in the mouse. Pulse-chase experiments in rat islets have demonstrated that the ratio between insulin I and II in newly synthesized insulin is higher than that of the stored insulin, indicating a slower conversion rate of proinsulin II compared to proinsulin I.

KW - Alleles

KW - Amino Acid Sequence

KW - Animals

KW - C-Peptide

KW - Chromatography, High Pressure Liquid

KW - Insulin

KW - Islets of Langerhans

KW - Mice

KW - Mice, Inbred Strains

KW - Molecular Sequence Data

KW - Proinsulin

KW - Rats

KW - Rats, Inbred Strains

M3 - Journal article

C2 - 2661585

VL - 462

SP - 243

EP - 254

JO - Journal of Chromatography A

JF - Journal of Chromatography A

SN - 0021-9673

ER -

ID: 47974424