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Reversible aggregation of lysozyme in a biodegradable amphiphilic multiblock copolymer

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningfagfællebedømt

  • van de Weert, Marco
  • Riemke van Dijkhuizen-Radersma
  • Jeroen M Bezemer
  • Wim E Hennink
  • Daan J A Crommelin
Lysozyme-loaded poly(ethylene glycol terephthalate)-poly(butylene terephthalate) (PEGT/PBT) films were prepared using a water-in-oil emulsification solvent evaporation method. Infrared spectroscopic analysis of the dried films indicated the presence of non-covalent lysozyme aggregates in the polymer matrix. The use of methanol to enhance the drying rate of the films increased the relative amount of aggregates. Surprisingly, quantitative in-vitro release of fully active, non-aggregated lysozyme was observed, indicating that lysozyme forms reversible aggregates during encapsulation in PEGT/PBT films.
OriginalsprogEngelsk
TidsskriftEuropean journal of pharmaceutics and biopharmaceutics : official journal of Arbeitsgemeinschaft fur Pharmazeutische Verfahrenstechnik e.V
Vol/bind54
Udgave nummer1
Sider (fra-til)89-93
Antal sider5
StatusUdgivet - jul. 2002
Eksternt udgivetJa

ID: 45184763