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Role of multicellular aggregates in biofilm formation

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Role of multicellular aggregates in biofilm formation. / Kragh, Kasper N.; Hutchison, Jaime B.; Melaugh, Gavin; Rodesney, Chris; Roberts, Aled E L; Irie, Yasuhiko; Jensen, Peter Østrup; Diggle, Stephen P.; Allen, Rosalind J.; Gordon, Vernita; Bjarnsholt, Thomas.

I: mBio, Bind 7, Nr. 2, e00237-16, 22.03.2016.

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningfagfællebedømt

Harvard

Kragh, KN, Hutchison, JB, Melaugh, G, Rodesney, C, Roberts, AEL, Irie, Y, Jensen, PØ, Diggle, SP, Allen, RJ, Gordon, V & Bjarnsholt, T 2016, 'Role of multicellular aggregates in biofilm formation' mBio, bind 7, nr. 2, e00237-16. https://doi.org/10.1128/mBio.00237-16

APA

Kragh, K. N., Hutchison, J. B., Melaugh, G., Rodesney, C., Roberts, A. E. L., Irie, Y., ... Bjarnsholt, T. (2016). Role of multicellular aggregates in biofilm formation. mBio, 7(2), [e00237-16]. https://doi.org/10.1128/mBio.00237-16

Vancouver

Kragh KN, Hutchison JB, Melaugh G, Rodesney C, Roberts AEL, Irie Y o.a. Role of multicellular aggregates in biofilm formation. mBio. 2016 mar 22;7(2). e00237-16. https://doi.org/10.1128/mBio.00237-16

Author

Kragh, Kasper N. ; Hutchison, Jaime B. ; Melaugh, Gavin ; Rodesney, Chris ; Roberts, Aled E L ; Irie, Yasuhiko ; Jensen, Peter Østrup ; Diggle, Stephen P. ; Allen, Rosalind J. ; Gordon, Vernita ; Bjarnsholt, Thomas. / Role of multicellular aggregates in biofilm formation. I: mBio. 2016 ; Bind 7, Nr. 2.

Bibtex

@article{22ebf026febb4b378ac8ab60ef72ce23,
title = "Role of multicellular aggregates in biofilm formation",
abstract = "In traditional models of in vitro biofilm development, individual bacterial cells seed a surface, multiply, and mature into multicellular, three-dimensional structures. Much research has been devoted to elucidating the mechanisms governing the initial attachment of single cells to surfaces. However, in natural environments and during infection, bacterial cells tend to clump as multicellular aggregates, and biofilms can also slough off aggregates as a part of the dispersal process. This makes it likely that biofilms are often seeded by aggregates and single cells, yet how these aggregates impact biofilm initiation and development is not known. Here we use a combination of experimental and computational approaches to determine the relative fitness of single cells and preformed aggregates during early development of Pseudomonas aeruginosa biofilms. We find that the relative fitness of aggregates depends markedly on the density of surrounding single cells, i.e., the level of competition for growth resources. When competition between aggregates and single cells is low, an aggregate has a growth disadvantage because the aggregate interior has poor access to growth resources. However, if competition is high, aggregates exhibit higher fitness, because extending vertically above the surface gives cells at the top of aggregates better access to growth resources. Other advantages of seeding by aggregates, such as earlier switching to a biofilm-like phenotype and enhanced resilience toward antibiotics and immune response, may add to this ecological benefit. Our findings suggest that current models of biofilm formation should be reconsidered to incorporate the role of aggregates in biofilm initiation.IMPORTANCE During the past decades, there has been a consensus around the model of development of a biofilm, involving attachment of single planktonic bacterial cells to a surface and the subsequent development of a mature biofilm. This study presents results that call for a modification of this rigorous model. We show how free floating biofilm aggregates can have a profound local effect on biofilm development when attaching to a surface. Our findings show that an aggregate landing on a surface will eventually outcompete the biofilm population arising from single cells attached around the aggregate and dominate the local biofilm development. These results point to a regime where preformed biofilm aggregates may have a fitness advantage over planktonic cells when it comes to accessing nutrients. Our findings add to the increasingly prominent comprehension that biofilm lifestyle is the default for bacteria and that planktonic single cells may be only a transition state at the most.",
author = "Kragh, {Kasper N.} and Hutchison, {Jaime B.} and Gavin Melaugh and Chris Rodesney and Roberts, {Aled E L} and Yasuhiko Irie and Jensen, {Peter {\O}strup} and Diggle, {Stephen P.} and Allen, {Rosalind J.} and Vernita Gordon and Thomas Bjarnsholt",
year = "2016",
month = "3",
day = "22",
doi = "10.1128/mBio.00237-16",
language = "English",
volume = "7",
journal = "mBio",
issn = "2161-2129",
publisher = "American Society for Microbiology",
number = "2",

}

RIS

TY - JOUR

T1 - Role of multicellular aggregates in biofilm formation

AU - Kragh, Kasper N.

AU - Hutchison, Jaime B.

AU - Melaugh, Gavin

AU - Rodesney, Chris

AU - Roberts, Aled E L

AU - Irie, Yasuhiko

AU - Jensen, Peter Østrup

AU - Diggle, Stephen P.

AU - Allen, Rosalind J.

AU - Gordon, Vernita

AU - Bjarnsholt, Thomas

PY - 2016/3/22

Y1 - 2016/3/22

N2 - In traditional models of in vitro biofilm development, individual bacterial cells seed a surface, multiply, and mature into multicellular, three-dimensional structures. Much research has been devoted to elucidating the mechanisms governing the initial attachment of single cells to surfaces. However, in natural environments and during infection, bacterial cells tend to clump as multicellular aggregates, and biofilms can also slough off aggregates as a part of the dispersal process. This makes it likely that biofilms are often seeded by aggregates and single cells, yet how these aggregates impact biofilm initiation and development is not known. Here we use a combination of experimental and computational approaches to determine the relative fitness of single cells and preformed aggregates during early development of Pseudomonas aeruginosa biofilms. We find that the relative fitness of aggregates depends markedly on the density of surrounding single cells, i.e., the level of competition for growth resources. When competition between aggregates and single cells is low, an aggregate has a growth disadvantage because the aggregate interior has poor access to growth resources. However, if competition is high, aggregates exhibit higher fitness, because extending vertically above the surface gives cells at the top of aggregates better access to growth resources. Other advantages of seeding by aggregates, such as earlier switching to a biofilm-like phenotype and enhanced resilience toward antibiotics and immune response, may add to this ecological benefit. Our findings suggest that current models of biofilm formation should be reconsidered to incorporate the role of aggregates in biofilm initiation.IMPORTANCE During the past decades, there has been a consensus around the model of development of a biofilm, involving attachment of single planktonic bacterial cells to a surface and the subsequent development of a mature biofilm. This study presents results that call for a modification of this rigorous model. We show how free floating biofilm aggregates can have a profound local effect on biofilm development when attaching to a surface. Our findings show that an aggregate landing on a surface will eventually outcompete the biofilm population arising from single cells attached around the aggregate and dominate the local biofilm development. These results point to a regime where preformed biofilm aggregates may have a fitness advantage over planktonic cells when it comes to accessing nutrients. Our findings add to the increasingly prominent comprehension that biofilm lifestyle is the default for bacteria and that planktonic single cells may be only a transition state at the most.

AB - In traditional models of in vitro biofilm development, individual bacterial cells seed a surface, multiply, and mature into multicellular, three-dimensional structures. Much research has been devoted to elucidating the mechanisms governing the initial attachment of single cells to surfaces. However, in natural environments and during infection, bacterial cells tend to clump as multicellular aggregates, and biofilms can also slough off aggregates as a part of the dispersal process. This makes it likely that biofilms are often seeded by aggregates and single cells, yet how these aggregates impact biofilm initiation and development is not known. Here we use a combination of experimental and computational approaches to determine the relative fitness of single cells and preformed aggregates during early development of Pseudomonas aeruginosa biofilms. We find that the relative fitness of aggregates depends markedly on the density of surrounding single cells, i.e., the level of competition for growth resources. When competition between aggregates and single cells is low, an aggregate has a growth disadvantage because the aggregate interior has poor access to growth resources. However, if competition is high, aggregates exhibit higher fitness, because extending vertically above the surface gives cells at the top of aggregates better access to growth resources. Other advantages of seeding by aggregates, such as earlier switching to a biofilm-like phenotype and enhanced resilience toward antibiotics and immune response, may add to this ecological benefit. Our findings suggest that current models of biofilm formation should be reconsidered to incorporate the role of aggregates in biofilm initiation.IMPORTANCE During the past decades, there has been a consensus around the model of development of a biofilm, involving attachment of single planktonic bacterial cells to a surface and the subsequent development of a mature biofilm. This study presents results that call for a modification of this rigorous model. We show how free floating biofilm aggregates can have a profound local effect on biofilm development when attaching to a surface. Our findings show that an aggregate landing on a surface will eventually outcompete the biofilm population arising from single cells attached around the aggregate and dominate the local biofilm development. These results point to a regime where preformed biofilm aggregates may have a fitness advantage over planktonic cells when it comes to accessing nutrients. Our findings add to the increasingly prominent comprehension that biofilm lifestyle is the default for bacteria and that planktonic single cells may be only a transition state at the most.

U2 - 10.1128/mBio.00237-16

DO - 10.1128/mBio.00237-16

M3 - Journal article

VL - 7

JO - mBio

JF - mBio

SN - 2161-2129

IS - 2

M1 - e00237-16

ER -

ID: 168885548