Forskning ved Københavns Universitet - Københavns Universitet


Rumen Microbiome Composition in Cattle during Grain-Induced Subacute Ruminal Acidosis (SARA)

Publikation: Bidrag til bog/antologi/rapportKonferenceabstrakt i proceedingsForskningfagfællebedømt

  • Anne Mette Danscher
  • Hooman Derakshani
  • Shucong Li
  • Jan C. Plaizier
  • Pia Haubro Andersen
  • Ehsan Khafipour
Six lactating, rumen cannulated, Danish Holstein cows were used in a blocked design
study including two blocks. In the first block, two cows received control diet and two cows
received SARA-challenge diet. In the second block, former control cows received SARAdiet
while two new cows received control diet. All cows received a total mixed ration
(TMR; 24% concentrate) for four weeks before the trial. SARA was induced by gradual
substitution of 40% of TMR with grain pellets (50:50 wheat:barley) over 3 days. Full SARAdiet
was fed for four days. Rumen samples were taken at 9 am and 9 pm on last day of
the control period, and first and last day of full SARA-feeding. DNA was extracted and V4
region of bacterial 16S rRNA amplified and subjected to Illumina sequencing. Data was
analyzed using QIIME pipelines and resultant operational taxonomic units (OTUs) were
aligned to Greengenes database. Differences in bacterial communities between groups
were tested based on weighted Unifrac distance using PERMANOVA and partial least
square discriminant analysis (PLS-DA). 556,536 high quality sequences were collected,
resulting in identification of 18,326 OTUs from 18 phyla of which 81 were classified at the
genus level. The rumen bacterial communities were altered in response to SARA (P=0.01).
The proportion of several taxa was significantly higher in SARA samples, including S24-7,
Erysipelotrichales. Lactobacillus, C lostridia, Moryella, Butyrivibrio, Olsenella, and
C oprococcus. Microbiome profiling of rumen during SARA could provide new knowledge of
the pathogenesis and might be used as a biological marker of the disease.
TitelRowett-INRA 2014 Gut Mircrobiology: From sequence to function
Antal sider1
Udgivelses stedAberdeen, Scotland, UK
Publikationsdato16 jun. 2014
StatusUdgivet - 16 jun. 2014

ID: 119236165