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Scalable and Purification-Free Synthesis of a Myristoylated Fluorogenic Sirtuin Substrate

Publikation: Bidrag til tidsskriftLetterForskningfagfællebedømt

Sirtuins are NAD+-dependent deacylases involved in the regulation of fundamental cellular processes in both normal and diseased cells. Therefore, the development of selective inhibitors for this class of enzymes is of great interest. Herein, we report an optimized synthesis of Ac-ETDK(myristoyl)-AMC, a sirtuin 2 substrate that can be used to profile inhibitors in fluorescence-based assays. The presented synthesis
is scalable and does not involve chromatographic purification, making this substrate readily available in few steps.
OriginalsprogEngelsk
TidsskriftSYNLETT: Accounts and Rapid Communications in Chemical Synthesis
Vol/bind28
Udgave nummer16
Sider (fra-til)2169-2173
ISSN0936-5214
DOI
StatusUdgivet - 2017

ID: 181197690