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Signal transduction by HLA-DR is mediated by tyrosine kinase(s) and regulated by CD45 in activated T cells

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Standard

Signal transduction by HLA-DR is mediated by tyrosine kinase(s) and regulated by CD45 in activated T cells. / Odum, Niels; Martin, P J; Schieven, G L; Norris, N A; Grosmaire, L S; Hansen, J A; Ledbetter, J A.

I: Human Immunology, Bind 32, Nr. 2, 1991, s. 85-94.

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningfagfællebedømt

Harvard

Odum, N, Martin, PJ, Schieven, GL, Norris, NA, Grosmaire, LS, Hansen, JA & Ledbetter, JA 1991, 'Signal transduction by HLA-DR is mediated by tyrosine kinase(s) and regulated by CD45 in activated T cells', Human Immunology, bind 32, nr. 2, s. 85-94.

APA

Odum, N., Martin, P. J., Schieven, G. L., Norris, N. A., Grosmaire, L. S., Hansen, J. A., & Ledbetter, J. A. (1991). Signal transduction by HLA-DR is mediated by tyrosine kinase(s) and regulated by CD45 in activated T cells. Human Immunology, 32(2), 85-94.

Vancouver

Odum N, Martin PJ, Schieven GL, Norris NA, Grosmaire LS, Hansen JA o.a. Signal transduction by HLA-DR is mediated by tyrosine kinase(s) and regulated by CD45 in activated T cells. Human Immunology. 1991;32(2):85-94.

Author

Odum, Niels ; Martin, P J ; Schieven, G L ; Norris, N A ; Grosmaire, L S ; Hansen, J A ; Ledbetter, J A. / Signal transduction by HLA-DR is mediated by tyrosine kinase(s) and regulated by CD45 in activated T cells. I: Human Immunology. 1991 ; Bind 32, Nr. 2. s. 85-94.

Bibtex

@article{e60b87e0fd9611ddb219000ea68e967b,
title = "Signal transduction by HLA-DR is mediated by tyrosine kinase(s) and regulated by CD45 in activated T cells",
abstract = "Recently, it was shown that HLA class II molecules on B cells and activated human T cells can transmit signals involving tyrosine phosphorylation of specific proteins, activation of the inositol phospholipid pathway, and release of cytosolic free Ca2+(Ca2+)i. The regulation of class II induced signals is poorly understood, however, and it remained unknown whether these pathways were coupled or activated independently. Here we show that a specific inhibitor of protein tyrosine kinases (PTK), herbimycin, abrogated DR-induced elevation of (Ca2+)i in activated human T cells. Genistein, belonging to another family of PTK inhibitors, had weaker but significant inhibitory effects on DR-induced (Ca2+)i responses. CD45 crosslinking with DR almost completely abrogated DR-induced (Ca2+)i responses and profoundly changed the PTK profiles. In contrast, CD4 crosslinking with DR enhanced the (Ca2+)i responses, but the inhibitory effect of CD45 dominated over the enhancing effect of CD4. These data indicate that PTK activation is obligatory for DR-induced (Ca2+)i responses, suggesting a linkage between these pathways in class II signal transduction. This conclusion is consistent with our observation that in activated human T cells, class II signals are up regulated by CD4, which is associated with p56lck, and down regulated by CD45, which is a tyrosine phosphatase.",
author = "Niels Odum and Martin, {P J} and Schieven, {G L} and Norris, {N A} and Grosmaire, {L S} and Hansen, {J A} and Ledbetter, {J A}",
note = "Keywords: Antigens, CD; Antigens, CD4; Antigens, CD45; Benzoquinones; Calcium; Cells, Cultured; Clone Cells; Enzyme Activation; HLA-DR Antigens; Histocompatibility Antigens; Humans; Lactams, Macrocyclic; Protein-Tyrosine Kinases; Quinones; Signal Transduction; T-Lymphocytes",
year = "1991",
language = "English",
volume = "32",
pages = "85--94",
journal = "Human Immunology",
issn = "0198-8859",
publisher = "Elsevier",
number = "2",

}

RIS

TY - JOUR

T1 - Signal transduction by HLA-DR is mediated by tyrosine kinase(s) and regulated by CD45 in activated T cells

AU - Odum, Niels

AU - Martin, P J

AU - Schieven, G L

AU - Norris, N A

AU - Grosmaire, L S

AU - Hansen, J A

AU - Ledbetter, J A

N1 - Keywords: Antigens, CD; Antigens, CD4; Antigens, CD45; Benzoquinones; Calcium; Cells, Cultured; Clone Cells; Enzyme Activation; HLA-DR Antigens; Histocompatibility Antigens; Humans; Lactams, Macrocyclic; Protein-Tyrosine Kinases; Quinones; Signal Transduction; T-Lymphocytes

PY - 1991

Y1 - 1991

N2 - Recently, it was shown that HLA class II molecules on B cells and activated human T cells can transmit signals involving tyrosine phosphorylation of specific proteins, activation of the inositol phospholipid pathway, and release of cytosolic free Ca2+(Ca2+)i. The regulation of class II induced signals is poorly understood, however, and it remained unknown whether these pathways were coupled or activated independently. Here we show that a specific inhibitor of protein tyrosine kinases (PTK), herbimycin, abrogated DR-induced elevation of (Ca2+)i in activated human T cells. Genistein, belonging to another family of PTK inhibitors, had weaker but significant inhibitory effects on DR-induced (Ca2+)i responses. CD45 crosslinking with DR almost completely abrogated DR-induced (Ca2+)i responses and profoundly changed the PTK profiles. In contrast, CD4 crosslinking with DR enhanced the (Ca2+)i responses, but the inhibitory effect of CD45 dominated over the enhancing effect of CD4. These data indicate that PTK activation is obligatory for DR-induced (Ca2+)i responses, suggesting a linkage between these pathways in class II signal transduction. This conclusion is consistent with our observation that in activated human T cells, class II signals are up regulated by CD4, which is associated with p56lck, and down regulated by CD45, which is a tyrosine phosphatase.

AB - Recently, it was shown that HLA class II molecules on B cells and activated human T cells can transmit signals involving tyrosine phosphorylation of specific proteins, activation of the inositol phospholipid pathway, and release of cytosolic free Ca2+(Ca2+)i. The regulation of class II induced signals is poorly understood, however, and it remained unknown whether these pathways were coupled or activated independently. Here we show that a specific inhibitor of protein tyrosine kinases (PTK), herbimycin, abrogated DR-induced elevation of (Ca2+)i in activated human T cells. Genistein, belonging to another family of PTK inhibitors, had weaker but significant inhibitory effects on DR-induced (Ca2+)i responses. CD45 crosslinking with DR almost completely abrogated DR-induced (Ca2+)i responses and profoundly changed the PTK profiles. In contrast, CD4 crosslinking with DR enhanced the (Ca2+)i responses, but the inhibitory effect of CD45 dominated over the enhancing effect of CD4. These data indicate that PTK activation is obligatory for DR-induced (Ca2+)i responses, suggesting a linkage between these pathways in class II signal transduction. This conclusion is consistent with our observation that in activated human T cells, class II signals are up regulated by CD4, which is associated with p56lck, and down regulated by CD45, which is a tyrosine phosphatase.

M3 - Journal article

C2 - 1835971

VL - 32

SP - 85

EP - 94

JO - Human Immunology

JF - Human Immunology

SN - 0198-8859

IS - 2

ER -

ID: 10636296