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Site-specific integrative elements of rhizobiophage 16-3 can integrate into proline tRNA (CGG) genes in different bacterial genera

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Standard

Site-specific integrative elements of rhizobiophage 16-3 can integrate into proline tRNA (CGG) genes in different bacterial genera. / Semsey, Szabolcs; Blaha, Béla; Köles, Krisztián; Orosz, László; Papp, Péter P.

I: Journal of Bacteriology, Bind 184, Nr. 1, 01.2002, s. 177-82.

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningfagfællebedømt

Harvard

Semsey, S, Blaha, B, Köles, K, Orosz, L & Papp, PP 2002, 'Site-specific integrative elements of rhizobiophage 16-3 can integrate into proline tRNA (CGG) genes in different bacterial genera', Journal of Bacteriology, bind 184, nr. 1, s. 177-82. https://doi.org/10.1128/JB.184.1.177-182.2002

APA

Semsey, S., Blaha, B., Köles, K., Orosz, L., & Papp, P. P. (2002). Site-specific integrative elements of rhizobiophage 16-3 can integrate into proline tRNA (CGG) genes in different bacterial genera. Journal of Bacteriology, 184(1), 177-82. https://doi.org/10.1128/JB.184.1.177-182.2002

Vancouver

Semsey S, Blaha B, Köles K, Orosz L, Papp PP. Site-specific integrative elements of rhizobiophage 16-3 can integrate into proline tRNA (CGG) genes in different bacterial genera. Journal of Bacteriology. 2002 jan;184(1):177-82. https://doi.org/10.1128/JB.184.1.177-182.2002

Author

Semsey, Szabolcs ; Blaha, Béla ; Köles, Krisztián ; Orosz, László ; Papp, Péter P. / Site-specific integrative elements of rhizobiophage 16-3 can integrate into proline tRNA (CGG) genes in different bacterial genera. I: Journal of Bacteriology. 2002 ; Bind 184, Nr. 1. s. 177-82.

Bibtex

@article{cc2030043c1b4a22bdf9a331d16ae7c1,
title = "Site-specific integrative elements of rhizobiophage 16-3 can integrate into proline tRNA (CGG) genes in different bacterial genera",
abstract = "The integrase protein of the Rhizobium meliloti 41 phage 16-3 has been classified as a member of the Int family of tyrosine recombinases. The site-specific recombination system of the phage belongs to the group in which the target site of integration (attB) is within a tRNA gene. Since tRNA genes are conserved, we expected that the target sequence of the site-specific recombination system of the 16-3 phage could occur in other species and integration could take place if the required putative host factors were also provided by the targeted cells. Here we report that a plasmid (pSEM167) carrying the attP element and the integrase gene (int) of the phage can integrate into the chromosomes of R. meliloti 1021 and eight other species. In all cases integration occurred at so-far-unidentified, putative proline tRNA (CGG) genes, indicating the possibility of their common origin. Multiple alignment of the sequences suggested that the location of the att core was different from that expected previously. The minimal attB was identified as a 23-bp sequence corresponding to the anticodon arm of the tRNA.",
keywords = "Bacteriophages, Base Sequence, DNA Transposable Elements, Molecular Sequence Data, Mutagenesis, Insertional, RNA, Transfer, Pro, Recombination, Genetic, Rhizobium, Rhodobacter sphaeroides, Sequence Homology, Nucleic Acid, Sinorhizobium meliloti, Species Specificity, Virus Integration, Journal Article, Research Support, Non-U.S. Gov't",
author = "Szabolcs Semsey and B{\'e}la Blaha and Kriszti{\'a}n K{\"o}les and L{\'a}szl{\'o} Orosz and Papp, {P{\'e}ter P}",
year = "2002",
month = "1",
doi = "10.1128/JB.184.1.177-182.2002",
language = "English",
volume = "184",
pages = "177--82",
journal = "Journal of Bacteriology",
issn = "0021-9193",
publisher = "American Society for Microbiology",
number = "1",

}

RIS

TY - JOUR

T1 - Site-specific integrative elements of rhizobiophage 16-3 can integrate into proline tRNA (CGG) genes in different bacterial genera

AU - Semsey, Szabolcs

AU - Blaha, Béla

AU - Köles, Krisztián

AU - Orosz, László

AU - Papp, Péter P

PY - 2002/1

Y1 - 2002/1

N2 - The integrase protein of the Rhizobium meliloti 41 phage 16-3 has been classified as a member of the Int family of tyrosine recombinases. The site-specific recombination system of the phage belongs to the group in which the target site of integration (attB) is within a tRNA gene. Since tRNA genes are conserved, we expected that the target sequence of the site-specific recombination system of the 16-3 phage could occur in other species and integration could take place if the required putative host factors were also provided by the targeted cells. Here we report that a plasmid (pSEM167) carrying the attP element and the integrase gene (int) of the phage can integrate into the chromosomes of R. meliloti 1021 and eight other species. In all cases integration occurred at so-far-unidentified, putative proline tRNA (CGG) genes, indicating the possibility of their common origin. Multiple alignment of the sequences suggested that the location of the att core was different from that expected previously. The minimal attB was identified as a 23-bp sequence corresponding to the anticodon arm of the tRNA.

AB - The integrase protein of the Rhizobium meliloti 41 phage 16-3 has been classified as a member of the Int family of tyrosine recombinases. The site-specific recombination system of the phage belongs to the group in which the target site of integration (attB) is within a tRNA gene. Since tRNA genes are conserved, we expected that the target sequence of the site-specific recombination system of the 16-3 phage could occur in other species and integration could take place if the required putative host factors were also provided by the targeted cells. Here we report that a plasmid (pSEM167) carrying the attP element and the integrase gene (int) of the phage can integrate into the chromosomes of R. meliloti 1021 and eight other species. In all cases integration occurred at so-far-unidentified, putative proline tRNA (CGG) genes, indicating the possibility of their common origin. Multiple alignment of the sequences suggested that the location of the att core was different from that expected previously. The minimal attB was identified as a 23-bp sequence corresponding to the anticodon arm of the tRNA.

KW - Bacteriophages

KW - Base Sequence

KW - DNA Transposable Elements

KW - Molecular Sequence Data

KW - Mutagenesis, Insertional

KW - RNA, Transfer, Pro

KW - Recombination, Genetic

KW - Rhizobium

KW - Rhodobacter sphaeroides

KW - Sequence Homology, Nucleic Acid

KW - Sinorhizobium meliloti

KW - Species Specificity

KW - Virus Integration

KW - Journal Article

KW - Research Support, Non-U.S. Gov't

U2 - 10.1128/JB.184.1.177-182.2002

DO - 10.1128/JB.184.1.177-182.2002

M3 - Journal article

C2 - 11741858

VL - 184

SP - 177

EP - 182

JO - Journal of Bacteriology

JF - Journal of Bacteriology

SN - 0021-9193

IS - 1

ER -

ID: 163918073