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Study of methicillin resistant Staphylococcus aureus (MRSA) in Danish pigs at slaughter and in imported retail meat reveals a novel MRSA type in slaughter pigs

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Standard

Study of methicillin resistant Staphylococcus aureus (MRSA) in Danish pigs at slaughter and in imported retail meat reveals a novel MRSA type in slaughter pigs. / Agersø, Yvonne; Hasman, Henrik; Cavaco, Lina M; Pedersen, Karl; Aarestrup, Frank M.

I: Veterinary Microbiology, Bind 157, Nr. 1-2, 25.05.2012, s. 246-50.

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningfagfællebedømt

Harvard

Agersø, Y, Hasman, H, Cavaco, LM, Pedersen, K & Aarestrup, FM 2012, 'Study of methicillin resistant Staphylococcus aureus (MRSA) in Danish pigs at slaughter and in imported retail meat reveals a novel MRSA type in slaughter pigs', Veterinary Microbiology, bind 157, nr. 1-2, s. 246-50. https://doi.org/10.1016/j.vetmic.2011.12.023

APA

Agersø, Y., Hasman, H., Cavaco, L. M., Pedersen, K., & Aarestrup, F. M. (2012). Study of methicillin resistant Staphylococcus aureus (MRSA) in Danish pigs at slaughter and in imported retail meat reveals a novel MRSA type in slaughter pigs. Veterinary Microbiology, 157(1-2), 246-50. https://doi.org/10.1016/j.vetmic.2011.12.023

Vancouver

Agersø Y, Hasman H, Cavaco LM, Pedersen K, Aarestrup FM. Study of methicillin resistant Staphylococcus aureus (MRSA) in Danish pigs at slaughter and in imported retail meat reveals a novel MRSA type in slaughter pigs. Veterinary Microbiology. 2012 maj 25;157(1-2):246-50. https://doi.org/10.1016/j.vetmic.2011.12.023

Author

Agersø, Yvonne ; Hasman, Henrik ; Cavaco, Lina M ; Pedersen, Karl ; Aarestrup, Frank M. / Study of methicillin resistant Staphylococcus aureus (MRSA) in Danish pigs at slaughter and in imported retail meat reveals a novel MRSA type in slaughter pigs. I: Veterinary Microbiology. 2012 ; Bind 157, Nr. 1-2. s. 246-50.

Bibtex

@article{7ea19f8ce36042c2a63df63d80b681c0,
title = "Study of methicillin resistant Staphylococcus aureus (MRSA) in Danish pigs at slaughter and in imported retail meat reveals a novel MRSA type in slaughter pigs",
abstract = "Methicillin-resistant Staphylococcus aureus (MRSA), especially CC398, have emerged in livestock worldwide. We investigated the occurrence of MRSA in pigs at slaughter and in retail meat. During 2009, nasal swabs (n=789) were taken from pigs at slaughter. Moreover, 866 meat samples [Danish: pork (153), broiler meat (121), beef (142) and; imported: pork (173), broiler meat (193), and beef (84)] were randomly collected in retail stores and outlets. MRSA was isolated from nasal swabs or from meat samples after preenrichment (Mueller Hinton broth with 6.5{\%} NaCl), selective enrichment (tryptone soya broth with 4 mg/L cefoxitine and 75 mg/L aztreonam) and selective plating on Brilliance Chromogenic MRSA agar. The presence of mecA was confirmed by PCR and the MRSA isolates were spa typed. Novel MRSA spa types were characterized by MLST, PFGE and SCCmec typing. Thirteen percent (101/789) of the pigs had MRSA. Based on spa types 93{\%} corresponded to CC398 (spa t011, t034, t1451, t2876, t2974), 4{\%} to CC30 (t1333) and one isolate to CC1 (t0127). The spa type t1333 (CC30), which is common among methicillin susceptible S. aureus (MSSA) from pigs in Denmark, contained a SCCmec cassette type V and czrC zinc resistance gene. Imported broiler meat had the highest occurrence (18{\%}) of MRSA, followed by imported pork (7.5{\%}) and Danish pork (4.6{\%}). MRSA ST398 was found for the first time in Danish beef (1.4{\%}). The finding of MRSA CC30 (spa t1333) suggest possible spread of the SCCmec cassette normally associated with ST398 into another S. aureus lineage common in pigs.",
keywords = "Animals, Anti-Bacterial Agents, Bacterial Typing Techniques, Cattle, Chickens, Denmark, Electrophoresis, Gel, Pulsed-Field, Food Contamination, Food Microbiology, Meat, Methicillin-Resistant Staphylococcus aureus, Microbial Sensitivity Tests, Multilocus Sequence Typing, Polymerase Chain Reaction, Sus scrofa, Journal Article, Research Support, Non-U.S. Gov't",
author = "Yvonne Agers{\o} and Henrik Hasman and Cavaco, {Lina M} and Karl Pedersen and Aarestrup, {Frank M}",
note = "Copyright {\circledC} 2012 Elsevier B.V. All rights reserved.",
year = "2012",
month = "5",
day = "25",
doi = "10.1016/j.vetmic.2011.12.023",
language = "English",
volume = "157",
pages = "246--50",
journal = "Veterinary Microbiology",
issn = "0378-1135",
publisher = "Elsevier",
number = "1-2",

}

RIS

TY - JOUR

T1 - Study of methicillin resistant Staphylococcus aureus (MRSA) in Danish pigs at slaughter and in imported retail meat reveals a novel MRSA type in slaughter pigs

AU - Agersø, Yvonne

AU - Hasman, Henrik

AU - Cavaco, Lina M

AU - Pedersen, Karl

AU - Aarestrup, Frank M

N1 - Copyright © 2012 Elsevier B.V. All rights reserved.

PY - 2012/5/25

Y1 - 2012/5/25

N2 - Methicillin-resistant Staphylococcus aureus (MRSA), especially CC398, have emerged in livestock worldwide. We investigated the occurrence of MRSA in pigs at slaughter and in retail meat. During 2009, nasal swabs (n=789) were taken from pigs at slaughter. Moreover, 866 meat samples [Danish: pork (153), broiler meat (121), beef (142) and; imported: pork (173), broiler meat (193), and beef (84)] were randomly collected in retail stores and outlets. MRSA was isolated from nasal swabs or from meat samples after preenrichment (Mueller Hinton broth with 6.5% NaCl), selective enrichment (tryptone soya broth with 4 mg/L cefoxitine and 75 mg/L aztreonam) and selective plating on Brilliance Chromogenic MRSA agar. The presence of mecA was confirmed by PCR and the MRSA isolates were spa typed. Novel MRSA spa types were characterized by MLST, PFGE and SCCmec typing. Thirteen percent (101/789) of the pigs had MRSA. Based on spa types 93% corresponded to CC398 (spa t011, t034, t1451, t2876, t2974), 4% to CC30 (t1333) and one isolate to CC1 (t0127). The spa type t1333 (CC30), which is common among methicillin susceptible S. aureus (MSSA) from pigs in Denmark, contained a SCCmec cassette type V and czrC zinc resistance gene. Imported broiler meat had the highest occurrence (18%) of MRSA, followed by imported pork (7.5%) and Danish pork (4.6%). MRSA ST398 was found for the first time in Danish beef (1.4%). The finding of MRSA CC30 (spa t1333) suggest possible spread of the SCCmec cassette normally associated with ST398 into another S. aureus lineage common in pigs.

AB - Methicillin-resistant Staphylococcus aureus (MRSA), especially CC398, have emerged in livestock worldwide. We investigated the occurrence of MRSA in pigs at slaughter and in retail meat. During 2009, nasal swabs (n=789) were taken from pigs at slaughter. Moreover, 866 meat samples [Danish: pork (153), broiler meat (121), beef (142) and; imported: pork (173), broiler meat (193), and beef (84)] were randomly collected in retail stores and outlets. MRSA was isolated from nasal swabs or from meat samples after preenrichment (Mueller Hinton broth with 6.5% NaCl), selective enrichment (tryptone soya broth with 4 mg/L cefoxitine and 75 mg/L aztreonam) and selective plating on Brilliance Chromogenic MRSA agar. The presence of mecA was confirmed by PCR and the MRSA isolates were spa typed. Novel MRSA spa types were characterized by MLST, PFGE and SCCmec typing. Thirteen percent (101/789) of the pigs had MRSA. Based on spa types 93% corresponded to CC398 (spa t011, t034, t1451, t2876, t2974), 4% to CC30 (t1333) and one isolate to CC1 (t0127). The spa type t1333 (CC30), which is common among methicillin susceptible S. aureus (MSSA) from pigs in Denmark, contained a SCCmec cassette type V and czrC zinc resistance gene. Imported broiler meat had the highest occurrence (18%) of MRSA, followed by imported pork (7.5%) and Danish pork (4.6%). MRSA ST398 was found for the first time in Danish beef (1.4%). The finding of MRSA CC30 (spa t1333) suggest possible spread of the SCCmec cassette normally associated with ST398 into another S. aureus lineage common in pigs.

KW - Animals

KW - Anti-Bacterial Agents

KW - Bacterial Typing Techniques

KW - Cattle

KW - Chickens

KW - Denmark

KW - Electrophoresis, Gel, Pulsed-Field

KW - Food Contamination

KW - Food Microbiology

KW - Meat

KW - Methicillin-Resistant Staphylococcus aureus

KW - Microbial Sensitivity Tests

KW - Multilocus Sequence Typing

KW - Polymerase Chain Reaction

KW - Sus scrofa

KW - Journal Article

KW - Research Support, Non-U.S. Gov't

U2 - 10.1016/j.vetmic.2011.12.023

DO - 10.1016/j.vetmic.2011.12.023

M3 - Journal article

C2 - 22245403

VL - 157

SP - 246

EP - 250

JO - Veterinary Microbiology

JF - Veterinary Microbiology

SN - 0378-1135

IS - 1-2

ER -

ID: 183246164