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Subclassification of cells in S phase in a partially synchronized cell system. Methodological problems concerning pulse labelling of epithelial cells in the hamster cheek pouch

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A circadian dependent delay in the incorporation of [ 3H]TdR into DNA, presumably due to variations in the intracellular pool of [ 3H]TdR derivatives, was found. It seems reasonable to relate this effect to a circadianally varying age distribution of cells in S phase. At any given time the S phase cells showed large variations in DNA synthesis rate, but it was still possible to identify a mean diurnal variation in the DNA synthesis rate. Differences in the ability of S phase cells to incorporate [ 3H]TdR are also discussed in relation to flow cytometrical measurements, and this contributes to the understanding of the commonly observed phenomenon that flow cytometry estimates of S-fractions are higher than those obtained with autoradiography.

OriginalsprogEngelsk
TidsskriftCell and Tissue Kinetics
Vol/bind15
Udgave nummer2
Sider (fra-til)157-168
Antal sider12
ISSN0008-8730
DOI
StatusUdgivet - mar. 1982

ID: 203011424