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Synthesis of an oligonucleotide-derivatized amphipol and its use to trap and immobilize membrane proteins

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningfagfællebedømt

  • Christel Le Bon
  • Eduardo Antonio Della Pia
  • Fabrice Giusti
  • Noemie Denise Carmen Lloret
  • Manuela Zoonens
  • Martinez, Karen Laurence
  • Jean Luc Popot

Amphipols (APols) are specially designed amphipathic polymers that stabilize membrane proteins (MPs) in aqueous solutions in the absence of detergent. A8-35, a polyacrylate-based APol, has been grafted with an oligodeoxynucleotide (ODN). The synthesis, purification and properties of the resulting 'OligAPol' have been investigated. Grafting was performed by reacting an ODN carrying an amine-terminated arm with the carboxylates of A8-35. The use of OligAPol for trapping MPs and immobilizing them onto solid supports was tested using bacteriorhodopsin (BR) and the transmembrane domain of Escherichia coli outer membrane protein A (tOmpA) as model proteins. BR and OligAPol form water-soluble complexes in which BR remains in its native conformation. Hybridization of the ODN arm with a complementary ODN was not hindered by the assembly of OligAPol into particles, nor by its association with BR. BR/OligAPol and tOmpA/OligAPol complexes could be immobilized onto either magnetic beads or gold nanoparticles grafted with the complementary ODN, as shown by spectroscopic measurements, fluorescence microscopy and the binding of anti-BR and anti-tOmpA antibodies. OligAPols provide a novel, highly versatile approach to tagging MPs, without modifying them chemically nor genetically, for specific, reversible and targetable immobilization, e.g. for nanoscale applications.

OriginalsprogEngelsk
Artikelnummere83
TidsskriftNucleic Acids Research
Vol/bind42
Udgave nummer10
Antal sider11
ISSN0305-1048
DOI
StatusUdgivet - 2014

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