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Synthesis of biologically active porcine secretin and [ITyr10] porcine secretin

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  • Hans Kofod
Porcine secretin, [Tyr10] secretin, and [Tyr13] secretin were synthesized by solid phase methodology and purified by stepwise gradient elution from a short reversed-phase column with ethanol and acetic acid as organic modifiers. [Tyr10] secretin and [Tyr13] secretin were iodinated by the chloramine-T method and nonmono-, and di-iodinated products separated and isolated by reversed-phase HPLC. Batch incubation analysis is isolated mouse pancreatic islets revealed that secretin and the [Tyr10] analogue were indistinguishable in their effect on the glucose-induced insulin release and cAMP accumulation. [Tyr13] secretin in contrast was significantly less potent in its effect on the glucose-induced insulin release.
OriginalsprogEngelsk
TidsskriftInternational Journal of Peptide and Protein Research
Vol/bind37
Udgave nummer3
Sider (fra-til)185-90
Antal sider6
StatusUdgivet - mar. 1991

ID: 45574735